Salmonella detection – the complete culture method

Every product for the classic Salmonella method, in the order of the method steps. Click a step to see all products for it in the catalogue.

1 · Pre-enrichmentNon-selective broth that resuscitates injured cells. 3 products →

2 · Selective enrichmentTwo parallel selective broths – RV and MKTTn. 14 products →

3 · PlatingTwo selective agars, e.g. XLD plus a second agar. 12 products →

4 · ConfirmationBiochemical and serological confirmation. 6 products →

+ · Rapid testsPlates, isothermal and qPCR kits for faster results. 3 products →

QC · QC strainsReference strains for media performance testing. 9 products →

Plating media for Salmonella

Selective agars for the isolation step. Most are also available granulated (250 g) – low dust and fast dissolution.

XLD AgarSKU PRO7437Red colonies with black centres. ISO, FDA, EP, USP.

Chromogenic Salmonella AgarSKU PRO7582Colour-coded colonies for fast differentiation.

Hektoen Enteric AgarSKU PRO7441Salmonella and Shigella from enteric samples.

Bismuth Sulfite AgarSKU PRO7467Highly selective, also for S. Typhi.

Brilliant Green AgarSKU PRO7482Second plating agar for Salmonella.

SS AgarSKU PRO7493Salmonella–Shigella agar for routine work.

MLCB AgarSKU PRO7558Detects H₂S-negative and lactose-positive strains.

XLT-4 Agar BaseSKU PRO7574For highly contaminated samples; with supplement.

Listeria detection

Enrichment in Half-Fraser and Fraser Broth, plating on a chromogenic agar of the Ottaviani-Agosti type plus PALCAM or Oxford Agar, and confirmation – as in ISO 11290-1. 30 products including supplements, rapid tests and the reference strain.

Half-Fraser Broth BaseSKU PRO7536Primary enrichment for Listeria.

Fraser Broth BaseSKU PRO7446Secondary enrichment; blackening shows Listeria.

Chromogenic Listeria AgarSKU PRO7594L. monocytogenes: blue-green with opaque halo.

PALCAM Agar BaseSKU PRO7470Second plating agar; grey-green with black halo.

TSYEASKU PRO7502Purification before confirmation.

L. monocytogenes LAMP KitSKU PRO7411Isothermal detection in about one hour.

Coliforms and E. coli

MPN broths, plating and chromogenic agars, water-testing media, rapid test papers, the IMViC identification kit and E. coli reference strains – for methods such as ISO 4831, ISO 4832, ISO 16649 and FDA BAM Chapter 4. 50 products.

Lauryl Sulfate Tryptose Broth (LST)SKU PRO7429Presumptive broth of the coliform MPN method.

Lauryl Sulfate Tryptose Broth (LST), granulatedSKU PRO7615Granules – less dust when weighing.

Lauryl Sulfate Tryptone Broth, granulatedSKU PRO7613Selective broth with sodium lauryl sulfate.

Lauryl Tryptose Broth with MUG (LST-MUG)SKU PRO7543Gas = coliforms; fluorescence = E. coli.

Modified Lauryl Tryptose Broth with MUGSKU PRO7544Fluorogenic MUG substrate for E. coli.

Brilliant Green Lactose Bile Broth (BGLB)SKU PRO7430Confirmation broth for coliforms.

Brilliant Green Lactose Bile Broth (BGLB), granulatedSKU PRO7616Granulated – cleaner weighing.

EC BrothSKU PRO7431Confirmation at 44.5 °C.

EC-MUG MediumSKU PRO7484E. coli: turbidity plus blue fluorescence.

MacConkey BrothSKU PRO7476Bile salts select Gram-negative bacteria.

MacConkey Broth PurpleSKU PRO7514Purple broth turns yellow with acid.

MacConkey Broth, granulatedSKU PRO7605Granulated format.

Lactose Bile Fermentation Broth, granulatedSKU PRO7606Single- and double-strength preparation.

Lactose Peptone Broth, granulatedSKU PRO7614Single, double or triple strength.

Membrane Lauryl Sulphate Broth (MLSB)SKU PRO7517For membrane filtration of water.

Presence-Absence BrothSKU PRO7520Qualitative test for 100 mL samples.

Violet Red Bile Lactose Agar (VRBL)SKU PRO7473Pour-plate count with overlay.

Violet Red Bile Agar (VRBA), granulatedSKU PRO7617Granulated – less dust.

Violet Red Bile Agar with MUG (VRBA-MUG)SKU PRO7504Coliforms: purple-red colonies.

MacConkey AgarSKU PRO7442Pink-red colonies = lactose fermenters.

MacConkey Agar No. 3SKU PRO7519Refined bile salts.

MacConkey Agar No. 1 without Crystal VioletSKU PRO7524No crystal violet – less inhibitory.

MacConkey Agar without SaltSKU PRO7534No NaCl – no Proteus swarming.

MacConkey Agar, granulatedSKU PRO7611Granulated – less dust.

Eosin Methylene Blue Agar (EMB)SKU PRO7457E. coli: black centre with green sheen.

Eosin Methylene Blue Agar (EMB), granulatedSKU PRO7607Granulated – cleaner weighing.

Deoxycholate AgarSKU PRO7509Sodium deoxycholate inhibits Gram-positives.

m-Endo AgarSKU PRO7503Membrane filtration method.

m-FC Agar with Rosolic AcidSKU PRO7491Membrane filtration at 44.5 °C.

Chromogenic Coliform AgarSKU PRO7579Coliforms: coloured colonies.

Chromogenic E. coli AgarSKU PRO7580E. coli: coloured colonies.

Chromogenic Coliform and E. coli AgarSKU PRO7581Two colours: coliforms and E. coli.

TBX Agar (Tryptone Bile X-glucuronide)SKU PRO7593Medium of ISO 16649 for E. coli counts.

Multiple Enzyme Substrate MediumSKU PRO7595Defined enzyme substrates.

Ampicillin MacConkey Agar SupplementSKU PRO77102 mg ampicillin per vial.

Lactose TTC Agar Supplement (Tergitol-7)SKU PRO7712Two components: Tergitol-7 and TTC.

Water Coliform Test Paper Type ISKU PRO7382No media preparation.

Water Coliform Test Paper Type IISKU PRO73835 × 10 mL portions per test.

Water Coliform Test Paper Type IIISKU PRO73849 papers per test, three volumes.

Clean Water Coliform Test Paper Type IVSKU PRO738615 papers per test.

Tableware Coliform Test PaperSKU PRO7385Pre-moistened – press onto the surface.

Tableware Coliform Test Paper Small PackSKU PRO738740 tests per box.

ONPG-MUG Medium for Coliforms and E. coliSKU PRO7823Yellow = coliforms (ONPG).

E. coli IMViC Identification KitSKU PRO7885Indole, MR, VP and citrate in one strip.

E. coli QC Strain CMCC(B) 44102SKU PRO7841Lyophilised, single use.

E. coli QC Strain CMCC(B) 44103SKU PRO7842Lyophilised, single use.

E. coli O157 QC Strain NCTC 12900SKU PRO7843Lyophilised, single use.

E. coli Quantitative QC Strain 10^3 (CMCC 44102)SKU PRO7866Lyophilised, single use.

E. coli Quantitative QC Strain 10^6 (CMCC 44102)SKU PRO7867Lyophilised, single use.

E. coli 8099 Cloth Carrier for Disinfection TestsSKU PRO7884Lyophilised, single use.

E. coli O157 and STEC

Sorbitol MacConkey and chromogenic agars with supplements, qPCR, LAMP and multiplex PCR kits, biochemical identification, latex and the O157 reference strain. 14 products.

Sorbitol MacConkey Agar Base (SMAC)SKU PRO7505O157: colourless colonies.

Sorbitol MacConkey Agar Supplement (CT)SKU PRO7687Potassium tellurite and cefixime.

Chromogenic E. coli O157 AgarSKU PRO7585O157: purple-red colonies.

Potassium Tellurite Supplement for E. coli O157 AgarSKU PRO7684Selective supplement for O157 agar.

Chromogenic STEC AgarSKU PRO7596O157 and non-O157 STEC.

E. coli O157 qPCR Detection Kit (O157:H7)SKU PRO7396Real-time PCR, FAM channel.

E. coli O157 LAMP Test Kit (O157:H7)SKU PRO7413Isothermal amplification at 64 °C.

STEC qPCR Detection KitSKU PRO7404stx1 and stx2 by real-time PCR.

EPEC STEC EHEC Multiplex PCR KitSKU PRO7422Multiplex end-point PCR.

EAEC Multiplex PCR KitSKU PRO7423Multiplex end-point PCR.

ETEC Multiplex PCR KitSKU PRO7424Multiplex end-point PCR.

EIEC Multiplex PCR KitSKU PRO7425Multiplex end-point PCR.

E. coli O157 Biochemical Identification KitSKU PRO788911-well biochemical strip.

E. coli O157 Latex KitSKU PRO7897Latex agglutination in minutes.

Count plates

Ready-to-use plates for hygiene indicators and spoilage organisms – a fast alternative to pour plates. 11 products.

Aerobic Count PlateSKU PRO7365Total viable count; rapid and membrane-filter versions.

E. coli & Coliform Count PlateSKU PRO7368E. coli and coliforms on one plate.

Enterobacteriaceae Count PlateSKU PRO7379Hygiene indicator for food and surfaces.

Mold & Yeast Count PlateSKU PRO7367Yeasts and moulds; rapid version available.

Lactic Acid Bacteria Count PlateSKU PRO7380Spoilage and starter-culture control.

Bacillus cereus Count PlateSKU PRO7381Presumptive B. cereus count.

Products by method step

More target organisms (E. coli, Staphylococcus, Cronobacter …) will be added step by step. Need a medium that is not listed? Send us the method – COLO.Science supplies the complete set.