E. coli · QC reference strain
E. coli 8099 Cloth Carrier – reference strain for media testing
The E. coli 8099 Cloth Carrier is a ready-made test carrier with 1–5 × 10^6 CFU of Escherichia coli 8099 per piece. It is used to evaluate the efficacy of disinfection in the field.
- Lyophilised, single use
- For growth-promotion and positive controls
- Rehydration fluid included
How E. coli 8099 Cloth Carrier is used
Carriers are placed at the test points before disinfection. After the contact time, each carrier is transferred to neutraliser, shaken, and the surviving bacteria are counted. The kill rate is calculated against untreated control carriers.
Applications and practical notes
Reference strains of E. coli are used for growth-promotion and selectivity testing of culture media according to ISO 11133 and for positive controls in routine methods. Typical applications include:
- Growth-promotion testing of new media lots
- Positive controls for coliform and E. coli methods
- Proficiency and method-verification studies
- Pharmacopoeial microbial limit tests
Working with E. coli 8099 Cloth Carrier: Always include untreated control carriers from the same lot to calculate the kill rate.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Salmonella culture method: the reference method for Salmonella in the food chain, ISO 6579-1, has four steps: non-selective pre-enrichment, selective enrichment in two broths, plating on two selective agars and confirmation of typical colonies. The FDA BAM Chapter 5 follows the same principle with media adapted to different food types. Rapid tests and identification kits shorten the time to result, but positive screening results are usually confirmed by the culture method. COLO.Science offers products for every step, so that one supplier covers the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
E. coli 8099 Cloth Carrier is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include E. coli 8099 Cloth Carrier, please send us a request. With every delivery of E. coli 8099 Cloth Carrier you receive the lot number and expiry date, and on request we send the available documentation for E. coli 8099 Cloth Carrier.
Where E. coli 8099 Cloth Carrier fits in coliform and E. coli testing
- Presumptive test – Lauryl Sulfate Tryptose Broth (MPN) or VRBL / chromogenic agar (colony count)
- Confirmation – BGLB Broth for coliforms; EC or EC-MUG Broth at 44.5 °C for E. coli
- Isolation – EMB, MacConkey, TBX or chromogenic E. coli agar
- Identification – IMViC kit; for O157 and STEC: SMAC or chromogenic O157 agar, latex and PCR
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| E. coli 8099 Cloth Carrier for Disinfection Tests | PRO7884 | 100 pcs |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
