Pseudomonas aeruginosa · Isothermal LAMP
Pseudomonas aeruginosa LAMP Test Kit – visual result in under one hour
The Pseudomonas aeruginosa LAMP Test Kit detects Pseudomonas aeruginosa DNA by loop-mediated isothermal amplification. The reaction runs at one temperature in a simple heating block, and the result is read by colour.
- Isothermal amplification at 64 °C
- Visual read-out: green = positive
- Detection limit about 8 CFU per test
How Pseudomonas aeruginosa LAMP Test Kit works
After enrichment and lysis at 99 °C, the sample is added to the lyophilised reaction tube. Specific primers amplify the target DNA in about 50 minutes. Positive reactions show green fluorescence, negative ones remain light orange.
Typical results
| Result | Read-out |
| Positive | Fluorescent green |
| Negative | Light orange |
Technical data
| Preparation | Lyophilised reagents; 20 µL reconstitution solution per reaction tube; sample lysis 99 °C, 10 min |
| Incubation | 64 °C (range 60–65 °C), 50 min |
| Storage | 2–8 °C |
| Shelf life | 12 months |
Applications and practical notes
Rapid molecular tests detect Pseudomonas aeruginosa in enrichment cultures of water and product samples within hours, while the culture method of ISO 16266 remains the reference. Typical applications include:
- Screening of water samples after membrane filtration
- Bottled water and beverage production
- Cosmetic and pharmaceutical products
- Laboratories with PCR or LAMP equipment
Working with Pseudomonas aeruginosa LAMP Test Kit: Open the reaction tubes only after the run in a separate area, or not at all, and run a positive and a negative control every time.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Pseudomonas methods: ISO 16266 describes the detection and enumeration of Pseudomonas aeruginosa in water by membrane filtration on CN agar, where blue-green colonies that produce pyocyanin are counted as confirmed and other typical colonies are confirmed with further tests. ISO 13720 gives the count of presumptive Pseudomonas spp. in meat and meat products on CFC agar at 25 °C. Pharmacopoeial tests for P. aeruginosa use Cetrimide Agar. COLO.Science offers media, supplements, rapid tests and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Pseudomonas aeruginosa LAMP Test Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Pseudomonas aeruginosa LAMP Test Kit, please send us a request. With every delivery of Pseudomonas aeruginosa LAMP Test Kit you receive the lot number and expiry date, and on request we send the available documentation for Pseudomonas aeruginosa LAMP Test Kit.
Where Pseudomonas aeruginosa LAMP Test Kit fits in Pseudomonas testing
- Water – P. aeruginosa – membrane filtration on CN agar (Pseudomonas Agar Base with CN supplement), 36 °C, 44 h
- Food – Pseudomonas spp. – CFC agar (Pseudomonas Agar Base with CFC supplement), 25 °C, 44 h
- Isolation and screening – Pseudomonas Chromogenic Medium and Cetrimide Agar
- Confirmation and QC – qPCR or LAMP and P. aeruginosa reference strains
See all Pseudomonas products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Pseudomonas aeruginosa LAMP Test Kit | PRO7405 | 24 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
