Vibrio · Real-time PCR
Vibrio parahaemolyticus qPCR Detection Kit – real-time PCR with fluorescent probe
The Vibrio parahaemolyticus qPCR Detection Kit detects Vibrio parahaemolyticus DNA by real-time PCR with a fluorescent probe after enrichment in alkaline saline peptone water. The kit contains ready-to-use reaction mix, lysis solution and controls.
- Real-time PCR, FAM channel
- Detection limit about 100 CFU per test
- Result within hours after enrichment
How Vibrio parahaemolyticus qPCR Detection Kit works
After enrichment, cells are lysed at 99 °C for 10 minutes and the DNA is amplified with specific primers and probe in 40 cycles. The Ct value shows whether the target is present.
Typical results
| Result | Read-out |
| Positive | Ct ≤ 35 |
| Retest | Ct 35–37 |
| Negative | Ct ≥ 37 or no Ct |
Technical data
| Sample preparation | Enrichment in 3 % NaCl alkaline peptone water, 36 ± 1 °C, 8–18 h; lysis 99 °C, 10 min |
| PCR program | 95 °C 30 s; 40 cycles of 95 °C 5 s / 60 °C 30 s |
| Storage | −20 °C, protected from light; avoid repeated freezing and thawing |
| Shelf life | 12 months |
Applications and practical notes
Rapid tests shorten the detection of Vibrio after enrichment, while positive results are confirmed by the culture method of ISO 21872-1. Typical applications include:
- Screening of seafood batches
- Aquaculture monitoring
- Laboratories that need results within a working day
- Confirmation of suspect colonies
Working with Vibrio parahaemolyticus qPCR Detection Kit: Open the reaction tubes only after the run in a separate area, or not at all, and run a positive and a negative control every time.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Vibrio method: ISO 21872-1 describes the detection of potentially enteropathogenic Vibrio parahaemolyticus, V. cholerae and V. vulnificus in seafood and other foods. Samples are enriched twice in Alkaline Saline Peptone Water, plated on TCBS Agar and a second selective medium, and typical colonies are purified on saline agar and confirmed biochemically or by PCR. The FDA BAM Chapter 9 follows a similar scheme. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Vibrio parahaemolyticus qPCR Detection Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Vibrio parahaemolyticus qPCR Detection Kit, please send us a request. With every delivery of Vibrio parahaemolyticus qPCR Detection Kit you receive the lot number and expiry date, and on request we send the available documentation for Vibrio parahaemolyticus qPCR Detection Kit.
Where Vibrio parahaemolyticus qPCR Detection Kit fits in Vibrio testing
- Enrichment – Alkaline Saline Peptone Water (ASPW), 37 °C and 41.5 °C
- Isolation – TCBS Agar plus a second medium: chromogenic Vibrio agar, mCPC or CC agar
- Purification – non-selective saline agar such as T1N1 Agar
- Confirmation – oxidase, biochemical identification, PCR or LAMP
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Vibrio parahaemolyticus qPCR Detection Kit | PRO7395 | 48 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
