Pseudomonas spp. · Supplement
CFC Supplement – selective supplement for CFC agar
The CFC Supplement turns Pseudomonas Agar Base into CFC agar for the count of presumptive Pseudomonas spp. in meat and meat products according to ISO 13720.
- Cetrimide, fucidin and a cephalosporin
- For CFC agar according to ISO 13720
- 10 vials
How CFC Supplement works
The combination of cetrimide, fucidin and a cephalosporin suppresses Gram-positive bacteria and most Enterobacteriaceae. Pseudomonas spp. grow at 25 °C, and oxidase-positive colonies are counted as presumptive Pseudomonas.
Applications and practical notes
Pseudomonas aeruginosa is an opportunistic pathogen of drinking, bottled and pool water, and Pseudomonas spp. are the main spoilage bacteria of chilled meat and fish. Typical applications according to ISO 16266 and ISO 13720 include:
- Drinking water, bottled and mineral water
- Swimming pool and spa water
- Meat, poultry and fish (spoilage flora)
- Pharmaceutical and cosmetic raw materials
Working with CFC Supplement: Reconstitute the vial as stated on the label, add it to the cooled base and confirm colonies with the oxidase test.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Pseudomonas methods: ISO 16266 describes the detection and enumeration of Pseudomonas aeruginosa in water by membrane filtration on CN agar, where blue-green colonies that produce pyocyanin are counted as confirmed and other typical colonies are confirmed with further tests. ISO 13720 gives the count of presumptive Pseudomonas spp. in meat and meat products on CFC agar at 25 °C. Pharmacopoeial tests for P. aeruginosa use Cetrimide Agar. COLO.Science offers media, supplements, rapid tests and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
CFC Supplement is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include CFC Supplement, please send us a request. With every delivery of CFC Supplement you receive the lot number and expiry date, and on request we send the available documentation for CFC Supplement.
Where CFC Supplement fits in Pseudomonas testing
- Water – P. aeruginosa – membrane filtration on CN agar (Pseudomonas Agar Base with CN supplement), 36 °C, 44 h
- Food – Pseudomonas spp. – CFC agar (Pseudomonas Agar Base with CFC supplement), 25 °C, 44 h
- Isolation and screening – Pseudomonas Chromogenic Medium and Cetrimide Agar
- Confirmation and QC – qPCR or LAMP and P. aeruginosa reference strains
See all Pseudomonas products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| CFC Supplement for Pseudomonas Agar | PRO7733 | 10 vials |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
