Yeasts and moulds · QC reference strain
Aspergillus brasiliensis QC Strain – reference strain for media testing
Aspergillus brasiliensis CMCC(F) 98003 (formerly listed as A. niger) is a qualitative reference mould for growth-promotion testing of fungal media and for the total yeast and mould count in pharmacopoeial tests.
- Lyophilised, single use
- For growth-promotion and controls
- Rehydration fluid included
How Aspergillus brasiliensis QC Strain works
The lyophilised culture is rehydrated, spread on Sabouraud Dextrose Agar or Potato Dextrose Agar and incubated at 20–25 °C until spores form. A spore suspension is then prepared for the test.
Technical data
| Preparation | Add 1.0 mL of the supplied recovery fluid, vortex and spread 0.1 mL on a non-selective plate or into broth |
| Incubation | 20–25 °C, 3–5 days |
| Storage | As stated on the label; use the rehydrated suspension within 8 hours (2–8 °C) |
Applications and practical notes
Reference strains of Candida albicans and Aspergillus brasiliensis are used for growth-promotion testing of fungal media according to ISO 11133 and in the pharmacopoeial tests EP 2.6.12 / USP <61>. Typical applications include:
- Growth-promotion testing of fungal media
- Positive controls in TYMC and C. albicans tests
- Method suitability (recovery) testing
- Proficiency and method-verification studies
Working with Aspergillus brasiliensis QC Strain: Open plates with sporulating mould only in a safety cabinet and prepare the spore suspension with a wetting agent such as polysorbate 80.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About yeast and mould methods: in the food chain, yeasts and moulds are counted by spread plating on DRBC Agar for products with a water activity above 0.95 according to ISO 21527-1, and on DG18 Agar for drier products according to ISO 21527-2. The FDA BAM Chapter 18 describes similar plate count methods. In pharmaceutical microbiology, the total yeast and mould count (TYMC) and the test for Candida albicans use Sabouraud media according to EP 2.6.12 / 2.6.13 and USP <61> / <62>. COLO.Science offers counting media, identification media, supplements and reference strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Aspergillus brasiliensis QC Strain is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Aspergillus brasiliensis QC Strain, please send us a request. With every delivery of Aspergillus brasiliensis QC Strain you receive the lot number and expiry date, and on request we send the available documentation for Aspergillus brasiliensis QC Strain.
Where Aspergillus brasiliensis QC Strain fits in yeast and mould testing
- Food and feed counts – DRBC Agar for aw > 0.95 (ISO 21527-1) and DG18 Agar for aw ≤ 0.95 (ISO 21527-2); alternatives: Rose Bengal Chloramphenicol, PDA with chloramphenicol, YGC for dairy products
- Pharmaceutical products – Sabouraud Dextrose Agar and Broth for TYMC and the Candida albicans test (EP 2.6.12 / 2.6.13, USP <61> / <62>)
- Cultivation and identification – PDA, Malt Extract and Czapek-Dox Agar for moulds; Corn Meal, chromogenic Candida and BIGGY Agar, Yeast Nitrogen Base for yeasts
- Fermentation and QC – WL Nutrient, Wort Agar and YPD for brewing and culture yeasts; C. albicans and A. brasiliensis reference strains for media testing
See the complete yeast and mould set in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Aspergillus brasiliensis QC Strain CMCC(F) 98003 | PRO7832 | 1 vial |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
