Burkholderia gladioli · Real-time PCR
Burkholderia gladioli qPCR Detection Kit – real-time PCR with fluorescent probe
The Burkholderia gladioli qPCR Detection Kit detects Burkholderia gladioli DNA by real-time PCR with a fluorescent probe after enrichment of the sample. The kit contains ready-to-use reaction mix, lysis solution and controls.
- Real-time PCR with fluorescent probe
- Detection limit about 100 CFU per test
- Result within hours after enrichment
How Burkholderia gladioli qPCR Detection Kit works
After enrichment, cells are lysed at 99 °C for 10 minutes and the DNA is amplified with specific primers and probe in 40 cycles. The Ct value shows whether the target is present.
Typical results
| Result | Read-out |
| Positive | Ct ≤ 35 |
| Retest | Ct 35–37 |
| Negative | Ct ≥ 37 or no Ct |
Technical data
| Sample preparation | Enrichment, 36 ± 1 °C, 20–24 h; lysis 99 °C, 10 min |
| PCR program | 40 cycles; fluorescence read at 60 °C in each cycle |
| Storage | −20 °C, protected from light; avoid repeated freezing and thawing |
| Shelf life | 12 months |
Applications and practical notes
Molecular kits detect Burkholderia gladioli and Brucella within hours, without long culture. Typical applications include:
- Fermented rice and corn products
- Edible fungi and starch products
- Milk and animal samples (Brucella)
- Laboratories with PCR or LAMP equipment
Working with Burkholderia gladioli qPCR Detection Kit: Open the reaction tubes only after the run in a separate area, or not at all, and run a positive and a negative control every time.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Burkholderia and Brucella methods: the USP chapter <60> describes the test for the Burkholderia cepacia complex in non-sterile pharmaceutical products, with enrichment and plating on Burkholderia cepacia selective agar (BCSA). Burkholderia gladioli, formerly known as Pseudomonas cocovenenans, produces bongkrekic acid in fermented cereal products and is isolated on PCFA agar. Brucella is detected in animal samples and milk mainly by molecular methods, because its culture is slow and needs a high biosafety level. COLO.Science offers supplements and rapid tests for these applications.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Burkholderia gladioli qPCR Detection Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Burkholderia gladioli qPCR Detection Kit, please send us a request. With every delivery of Burkholderia gladioli qPCR Detection Kit you receive the lot number and expiry date, and on request we send the available documentation for Burkholderia gladioli qPCR Detection Kit.
Where Burkholderia gladioli qPCR Detection Kit fits in Burkholderia and Brucella testing
- Burkholderia cepacia complex – BCSA agar with selective supplement, for non-sterile products and water
- Burkholderia gladioli – PCFA agar with selective supplement, for fermented foods
- Fast screening – qPCR or LAMP kits for B. gladioli
- Brucella – qPCR or LAMP kits for animal and milk samples
See all Burkholderia and Brucella products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Burkholderia gladioli qPCR Detection Kit | PRO7403 | 48 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
