Bacillus cereus · QC reference strain
Bacillus cereus QC Strain – CMCC(B) 63303 reference strain
Bacillus cereus CMCC(B) 63303 is the strain used to check the productivity of MYP Agar and Chromogenic Bacillus cereus Agar: it forms pink colonies with a precipitate zone on MYP.
- Lyophilised, single use
- For growth-promotion and controls
- Recovery fluid included
How Bacillus cereus QC Strain is used
After rehydration and subculture, the strain is inoculated on the new media lot together with a negative control such as Escherichia coli, and colony appearance and recovery are recorded.
Technical data
| Preparation | Add 1.0 mL of the supplied recovery fluid, vortex and spread 0.1 mL on a non-selective plate or into broth |
| Incubation | 36 ± 1 °C, 24–48 h |
| Storage | As stated on the label; use the rehydrated suspension within 8 hours (2–8 °C) |
Applications and practical notes
Reference strains of Bacillus are used for growth-promotion and selectivity testing of media according to ISO 11133 and as positive controls in routine methods. Typical applications include:
- Growth-promotion testing of MYP and chromogenic agars
- Positive controls for Bacillus cereus methods
- Method verification and proficiency testing
- Disinfectant and spore tests
Working with Bacillus cereus QC Strain: Subculture the strain no more than five times from the original vial and record the passage number.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Bacillus cereus method: presumptive Bacillus cereus is counted according to ISO 7932 on Mannitol Egg Yolk Polymyxin (MYP) Agar: typical colonies are pink (mannitol not fermented) and surrounded by a precipitate of hydrolysed lecithin. Selected colonies are confirmed by haemolysis on blood agar. The FDA BAM Chapter 14 adds tests that separate B. cereus from B. thuringiensis by its toxin crystals. COLO.Science offers media, supplements, identification kits and QC strains for the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Bacillus cereus QC Strain is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Bacillus cereus QC Strain, please send us a request. With every delivery of Bacillus cereus QC Strain you receive the lot number and expiry date, and on request we send the available documentation for Bacillus cereus QC Strain.
Where Bacillus cereus QC Strain fits in Bacillus cereus testing
- Sample preparation – dilution in Buffered Peptone Water or peptone saline
- Plate count – MYP Agar with polymyxin B and egg yolk, 30 °C, 18–48 h
- Alternative isolation – Chromogenic Bacillus cereus Agar or Bacillus cereus Count Plate
- Confirmation – haemolysis, biochemical identification, protein crystal stain; QC strains for each run
See all Bacillus cereus products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Bacillus cereus QC Strain CMCC(B) 63303 | PRO7834 | 1 vial |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
