Cronobacter · Real-time PCR
Cronobacter qPCR Detection Kit – real-time PCR with fluorescent probe
The Cronobacter qPCR Detection Kit detects Cronobacter sakazakii DNA by real-time PCR with a fluorescent probe after enrichment. The kit contains ready-to-use reaction mix, lysis solution and controls.
- Real-time PCR with fluorescent probe
- Detection limit about 100 CFU per test
- Result within hours after enrichment
How Cronobacter qPCR Detection Kit works
After enrichment, cells are lysed at 99 °C for 10 minutes and the DNA is amplified with specific primers and probe in 40 cycles. The Ct value shows whether the target is present.
Typical results
| Result | Read-out |
| Positive | Ct ≤ 35 |
| Retest | Ct 35–37 |
| Negative | Ct ≥ 37 or no Ct |
Technical data
| Sample preparation | Enrichment in Buffered Peptone Water, 36 ± 1 °C, 18–24 h; lysis 99 °C, 10 min |
| PCR program | 95 °C 30 s; 40 cycles of 95 °C 5 s / 60 °C 30 s |
| Storage | −20 °C, protected from light; avoid repeated freezing and thawing |
| Shelf life | 12 months |
Applications and practical notes
Rapid tests shorten the time to result for Cronobacter after enrichment, while positive results are confirmed by the culture method of ISO 22964. Typical applications include:
- Screening of infant formula batches before release
- Environmental monitoring in powder plants
- Laboratories that need results within a working day
- Confirmation of suspect colonies
Working with Cronobacter qPCR Detection Kit: Separate the areas for sample preparation and PCR, and run a positive and a negative control in every run.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Cronobacter method: ISO 22964 is the horizontal method for Cronobacter spp. in infant formula, infant cereals, milk powders and production environments. It combines pre-enrichment in Buffered Peptone Water, selective enrichment at 41.5 °C, plating on a chromogenic agar and biochemical confirmation; the FDA BAM Chapter 29 uses a similar culture and PCR approach. COLO.Science offers media, supplements, rapid tests and identification kits for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Cronobacter qPCR Detection Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Cronobacter qPCR Detection Kit, please send us a request. With every delivery of Cronobacter qPCR Detection Kit you receive the lot number and expiry date, and on request we send the available documentation for Cronobacter qPCR Detection Kit.
Where Cronobacter qPCR Detection Kit fits in Cronobacter testing
- Pre-enrichment – Buffered Peptone Water, 34–38 °C
- Selective enrichment – Cronobacter enrichment broth with vancomycin-type supplement at 41.5 °C
- Isolation – Chromogenic Cronobacter Agar: blue-green colonies
- Confirmation – biochemical identification; rapid screening by qPCR, LAMP or detection plate
See all Cronobacter products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Cronobacter qPCR Detection Kit | PRO7397 | 48 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
