Pharmacopoeial media · Sterility test and enrichment · granulated
Tryptic Soy Broth – pharmacopoeial formulation, granulated
This granulated Tryptic Soy Broth has the soya-bean casein digest medium formulation of the pharmacopoeias. It is used for the sterility test, as the enrichment broth in tests for specified micro-organisms and for dilution of samples in microbial limit testing.
- Granulated – less dust, better flow
- Pharmacopoeial soya-bean casein digest medium
- Sterility test, enrichment and dilution
How Tryptic Soy Broth works
The peptone base with glucose supports aerobic bacteria and fungi. Growth of the pharmacopoeial reference strains, as turbidity for bacteria and as visible growth for yeasts and moulds, is checked on every lot.
Typical growth
| Organism | Growth |
| Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli | Turbid growth |
| Candida albicans | Growth within 3 days |
| Aspergillus brasiliensis | Growth within 5 days |
Technical data
| Preparation | 30 g/L; boil to dissolve, dispense, autoclave 121 °C 15 min |
| Final pH | 7.3 ± 0.2 |
| Incubation | Bacteria 30–35 °C, 18–24 h; fungi 20–25 °C, 2–5 days |
| Storage | Dry, dark place; keep the container tightly closed |
| Shelf life | 3 years unopened |
Applications and practical notes
The sterility test of the European Pharmacopoeia (chapter 2.6.1) and USP <71> uses two media: fluid thioglycollate medium for anaerobic and aerobic bacteria and soya-bean casein digest medium for aerobic bacteria and fungi, each incubated for 14 days. Typical applications include:
- Sterility testing of injectables and sterile products
- Media fill (aseptic process simulation)
- Sterility of medical devices and raw materials
- Growth promotion testing of each batch
Working with Tryptic Soy Broth: Weigh the granules without grinding them and keep the bottle closed between uses, because granules also absorb moisture.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About pharmacopoeial microbiological testing: the European Pharmacopoeia and the USP describe harmonised methods for non-sterile products – microbial enumeration (Ph. Eur. 2.6.12, USP <61>) and tests for specified micro-organisms (Ph. Eur. 2.6.13, USP <62>) – and the sterility test for sterile products (Ph. Eur. 2.6.1, USP <71>). The chapters define the media, incubation conditions and the growth promotion test that each batch of medium must pass with reference strains. COLO.Science offers the media, diluents, neutralisers and QC strains for these tests from one source.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Tryptic Soy Broth is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Tryptic Soy Broth, please send us a request. With every delivery of Tryptic Soy Broth you receive the lot number and expiry date, and on request we send the available documentation for Tryptic Soy Broth.
Where Tryptic Soy Broth fits in pharmacopoeial testing
- Sample preparation – Buffered Sodium Chloride-Peptone Solution, Tryptic Soy Broth and neutralising diluents
- Microbial enumeration – Tryptic Soy Agar (TAMC) and Sabouraud Dextrose Agar (TYMC)
- Specified micro-organisms – enrichment in Tryptic Soy Broth, then MacConkey, Mannitol Salt, Cetrimide, XLD, VRBG, RCM and Columbia media
- Sterility test – Fluid Thioglycollate Medium and Tryptic Soy Broth, 14 days
See all pharmacopoeial media in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Tryptic Soy Broth (Pharmacopoeia), granulated | PRO7624 | 250 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
