Cronobacter · Supplement
mLST Broth Supplement – glycopeptide supplement for Cronobacter enrichment
The mLST Broth Supplement contains norvancomycin, a glycopeptide antibiotic closely related to vancomycin, for completing Modified Lauryl Sulfate Tryptose Broth. One vial is added to 100 mL of cooled broth.
- 1 mg norvancomycin per vial
- One vial per 100 mL of mLST broth
- Suppresses Gram-positive bacteria
How mLST Broth Supplement works
Glycopeptide antibiotics bind to the cell wall precursors of Gram-positive bacteria and stop their growth, while Gram-negative Cronobacter are not affected. The supplement therefore improves the selectivity of the enrichment broth.
Technical data
| Composition | Norvancomycin 1 mg per vial, lyophilised |
| Use | Dissolve in 1 mL sterile saline and add to 100 mL of cooled broth |
| Storage | 2–8 °C |
| Shelf life | 2 years |
Applications and practical notes
Cronobacter spp. (formerly Enterobacter sakazakii) can cause severe infections in newborns, so powdered infant formula and its production environment are tested according to ISO 22964. Typical applications include:
- Powdered infant formula and follow-on formula
- Infant cereals and milk powders
- Environmental samples from dry production areas
- Ingredients for infant foods
Working with mLST Broth Supplement: Dissolve the vial contents in 1 mL of sterile saline just before use and add them aseptically to broth cooled to about 30 °C.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Cronobacter method: ISO 22964 is the horizontal method for Cronobacter spp. in infant formula, infant cereals, milk powders and production environments. It combines pre-enrichment in Buffered Peptone Water, selective enrichment at 41.5 °C, plating on a chromogenic agar and biochemical confirmation; the FDA BAM Chapter 29 uses a similar culture and PCR approach. COLO.Science offers media, supplements, rapid tests and identification kits for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
mLST Broth Supplement is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include mLST Broth Supplement, please send us a request. With every delivery of mLST Broth Supplement you receive the lot number and expiry date, and on request we send the available documentation for mLST Broth Supplement.
Where mLST Broth Supplement fits in Cronobacter testing
- Pre-enrichment – Buffered Peptone Water, 34–38 °C
- Selective enrichment – Cronobacter enrichment broth with vancomycin-type supplement at 41.5 °C
- Isolation – Chromogenic Cronobacter Agar: blue-green colonies
- Confirmation – biochemical identification; rapid screening by qPCR, LAMP or detection plate
See all Cronobacter products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| mLST Broth Supplement | PRO7682 | 10 vials |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
