Campylobacter · Supplement
Karmali Agar Supplement – two-component supplement for Karmali agar
The Karmali Agar Supplement completes Karmali agar, a blood-free selective medium for thermotolerant Campylobacter that is often used as the second isolation medium in ISO 10272-1. It consists of component A and component B.
- Components A and B
- Blood-free Karmali agar
- 1 A + 1 B per 100 mL
How Karmali Agar Supplement works
Component A contains sodium pyruvate, vancomycin, polymyxin B, amphotericin B and rifampicin; component B contains cefoperazone. Together they suppress bacteria and fungi, while pyruvate protects Campylobacter from oxygen toxicity.
Typical colony appearance
| Organism | Colonies |
| Campylobacter jejuni | Smooth, slightly convex greyish-white colonies |
| Staphylococcus aureus | Inhibited |
Technical data
| Composition | A: sodium pyruvate 10 mg, vancomycin 2 mg, polymyxin B 1 mg, amphotericin B 1 mg, rifampicin 0.625 mg; B: cefoperazone 3.2 mg (per vial) |
| Use | Dissolve each in 1 mL sterile saline; 1 A + 1 B per 100 mL base at about 50 °C |
| Incubation | 41–43 °C, microaerobic, 40–48 h |
| Storage | 2–8 °C |
Applications and practical notes
Campylobacter is the most frequently reported cause of bacterial gastroenteritis in Europe and is tested in poultry, meat, milk and water according to ISO 10272-1. Typical applications include:
- Poultry meat and carcass rinses
- Raw milk and dairy products
- Drinking and surface water
- Environmental and faecal samples
Working with Karmali Agar Supplement: Dissolve each vial in 1 mL of sterile saline and add one vial A and one vial B to 100 mL of sterile base at about 50 °C.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Campylobacter method: ISO 10272-1 describes the detection of thermotolerant Campylobacter in food and feed. Samples with low numbers and stressed cells are enriched in Bolton broth, samples with high background flora in Preston broth. Enrichment cultures are plated on mCCD agar and a second selective agar of the laboratory’s choice and incubated microaerobically at 41.5 °C. Typical colonies are confirmed by morphology, motility, oxidase and further tests. COLO.Science offers supplements, media and latex kits for these steps.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Karmali Agar Supplement is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Karmali Agar Supplement, please send us a request. With every delivery of Karmali Agar Supplement you receive the lot number and expiry date, and on request we send the available documentation for Karmali Agar Supplement.
Where Karmali Agar Supplement fits in Campylobacter testing
- Enrichment – Bolton broth or Preston broth with selective supplement, microaerobic
- Selective isolation – mCCD agar plus a second medium such as Skirrow or Karmali agar, 41.5 °C
- Confirmation – morphology, oxidase and latex agglutination
- Media QC – Campylobacter reference strains
See all Campylobacter products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Karmali Agar Supplement | PRO7726 | 2 × 5 vials |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
