Clostridium · QC reference strain
Clostridium sporogenes QC Strain – CMCC(B) 64941 reference strain
Clostridium sporogenes CMCC(B) 64941 is a qualitative anaerobic reference strain. It is used for growth-promotion testing of Fluid Thioglycollate Medium and Reinforced Clostridial Medium and for controls of anaerobic incubation.
- Anaerobic reference strain
- For FTM, RCM and anaerobic media
- Lyophilised, single use
How Clostridium sporogenes QC Strain is used
The lyophilised culture is rehydrated with 1 mL of the supplied fluid and grown in an anaerobic medium or on agar under anaerobic conditions for 48–96 hours. Purity is checked before use.
Technical data
| Preparation | Add 1.0 mL of the supplied recovery fluid, vortex and inoculate an anaerobic medium |
| Incubation | 36 ± 1 °C, anaerobic, 48–96 h |
| Storage | As stated on the label; use the rehydrated suspension within 8 hours (2–8 °C) |
Applications and practical notes
Clostridium sporogenes is a reference strain for anaerobic growth in culture media and in the sterility test of the pharmacopoeia, and for media testing according to ISO 11133. Typical applications include:
- Growth-promotion testing of Fluid Thioglycollate Medium
- Pharmacopoeial sterility and microbial limit tests
- Anaerobic media and jar controls
- Method verification
Working with Clostridium sporogenes QC Strain: Incubate the strain anaerobically and subculture it no more than five times from the original vial.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Clostridium methods: Clostridium perfringens in food and feed is counted according to ISO 7937 on Tryptose Sulfite Cycloserine (TSC) Agar under anaerobic conditions, where sulfite reduction gives black colonies, and ISO 14189 describes the membrane filtration method for water. Sulfite-reducing clostridia are counted on iron sulfite agar according to ISO 15213-1. COLO.Science offers media, supplements, rapid tests and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Clostridium sporogenes QC Strain is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Clostridium sporogenes QC Strain, please send us a request. With every delivery of Clostridium sporogenes QC Strain you receive the lot number and expiry date, and on request we send the available documentation for Clostridium sporogenes QC Strain.
Where Clostridium sporogenes QC Strain fits in Clostridium testing
- Plate count – TSC Agar with D-cycloserine, anaerobic, 37 °C, 20 h
- Sulfite-reducing clostridia – Iron Sulphite Agar and supplements, anaerobic
- Confirmation – nitrate, lactose, gelatin and motility tests; PCR or LAMP
- Quality control – Clostridium sporogenes reference strains
See all Clostridium products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Clostridium sporogenes QC Strain CMCC(B) 64941 | PRO7839 | 1 vial |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
