Yersinia enterocolitica · Real-time PCR
Yersinia enterocolitica qPCR Detection Kit – real-time PCR with fluorescent probe
The Yersinia enterocolitica qPCR Detection Kit detects Yersinia enterocolitica DNA by real-time PCR with a fluorescent probe after enrichment of the sample.
- Real-time PCR with fluorescent probe
- Ready-to-use reaction mix and controls
- 48 tests
How Yersinia enterocolitica qPCR Detection Kit works
After enrichment, the cells are lysed and the DNA is amplified with specific primers and probe. The Ct value shows whether the target is present; positive and negative controls are run in every series.
Applications and practical notes
Rapid tests, identification kits and reference strains support the detection of Yersinia enterocolitica according to ISO 10273. Typical applications include:
- Screening of enrichment cultures
- Confirmation of typical colonies on CIN agar
- Positive controls and media QC
- Laboratories with PCR or LAMP equipment
Working with Yersinia enterocolitica qPCR Detection Kit: Open the reaction tubes only after the run in a separate area, or not at all, and run a positive and a negative control every time.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Shigella and Yersinia methods: ISO 21567 describes the detection of Shigella in food by enrichment in Shigella broth with novobiocin, plating on selective agars such as MacConkey, XLD and Hektoen enteric agar, and biochemical and serological confirmation. ISO 10273 describes the detection of presumptive pathogenic Yersinia enterocolitica by enrichment at 25 °C and plating on CIN agar, followed by tests that separate pathogenic from non-pathogenic strains. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Yersinia enterocolitica qPCR Detection Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Yersinia enterocolitica qPCR Detection Kit, please send us a request. With every delivery of Yersinia enterocolitica qPCR Detection Kit you receive the lot number and expiry date, and on request we send the available documentation for Yersinia enterocolitica qPCR Detection Kit.
Where Yersinia enterocolitica qPCR Detection Kit fits in Shigella and Yersinia testing
- Enrichment – Shigella broth with selective supplement; for Yersinia a peptone-sorbitol-bile type broth
- Selective isolation – Chromogenic Shigella Agar, Desoxycholate Citrate Agar, XLD; for Yersinia CIN agar and Chromogenic Yersinia enterocolitica Agar
- Screening – qPCR or LAMP kits after enrichment
- Confirmation and QC – biochemical identification kits and reference strains
See all Shigella and Yersinia products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Yersinia enterocolitica qPCR Detection Kit | PRO7398 | 48 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
