Hygiene indicators · count plate
E. coli and Coliform Count Plate – ready-to-use plate for E. coli and coliform bacteria on one plate
The E. coli and Coliform Count Plate is a ready-to-use dry film plate for E. coli and coliform bacteria on one plate. No media preparation, pouring or melting of agar is needed – the sample is applied, incubated and the colonies are counted directly on the plate.
- Ready to use, no media preparation
- Results read directly on the plate
- Pack of 20 plates · COLO code PRO7368
How the E. coli and Coliform Count Plate works
Two chromogenic substrates differentiate the colonies: β-glucuronidase-positive E. coli forms blue-green colonies, while other coliforms form magenta colonies.
Reading the plate
| Colonies | What to count |
| Blue-green colonies | E. coli |
| Magenta colonies | other coliforms |
Count plates with a well-countable number of colonies and multiply the result by the dilution factor.
Technical data
| Sample preparation | 1 : 10 suspension in phosphate buffer or saline, serial dilutions; 1 mL per plate |
| Incubation | 36 ± 1 °C, 18–24 h, face up |
| Storage | 2–8 °C, sealed; after opening reseal the bag and use within 4 weeks |
| Shelf life | 18 months |
Applications and practical notes
The E. coli and Coliform Count Plate is used in routine hygiene control and product testing in food, beverage and water laboratories. Typical applications include:
- raw materials and finished products
- surface and hygiene monitoring with swabs
- water and beverages
- shelf-life studies
Working with E. coli and Coliform Count Plate: Lift the top film, dispense 1 mL in the centre of the plate and lower the film slowly to avoid air bubbles. Incubate the plates face up and do not stack more than 20 plates.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Salmonella culture method: the reference method for Salmonella in the food chain, ISO 6579-1, has four steps: non-selective pre-enrichment, selective enrichment in two broths, plating on two selective agars and confirmation of typical colonies. The FDA BAM Chapter 5 follows the same principle with media adapted to different food types. Rapid tests and identification kits shorten the time to result, but positive screening results are usually confirmed by the culture method. COLO.Science offers products for every step, so that one supplier covers the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
E. coli and Coliform Count Plate is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include E. coli and Coliform Count Plate, please send us a request. With every delivery of E. coli and Coliform Count Plate you receive the lot number and expiry date, and on request we send the available documentation for E. coli and Coliform Count Plate.
Where the E. coli and Coliform Count Plate fits in the counting procedure
- Sample preparation – 1 : 10 initial suspension in sterile diluent
- Dilution – ten-fold serial dilutions as needed
- Inoculation – 1 mL per plate, spread by the gel
- Incubation and counting – count the typical colonies and calculate CFU per g or mL
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| E. coli and Coliform Count Plate | PRO7368 | 20 plates |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
