Yersinia · Selective isolation
CIN Agar Base – selective agar for Yersinia
CIN Agar Base is the base for cefsulodin-irgasan-novobiocin (CIN) agar, the selective medium for the isolation of Yersinia from food, water and clinical samples. It is completed with the CIN Agar Supplement.
- Base for CIN (Yersinia selective) agar
- Mannitol and neutral red
- Completed with CIN Agar Supplement
How CIN Agar Base works
Sodium deoxycholate and crystal violet inhibit Gram-positive bacteria, and the antibiotics of the supplement suppress most Gram-negative flora. Yersinia ferments mannitol and forms small colonies with a deep red centre and a transparent edge – the typical “bull’s eye”.
Typical colony appearance
| Organism | Colonies |
| Yersinia enterocolitica | Small colonies with deep red centre and transparent edge |
Technical data
| Preparation | 57 g/L; heat with stirring to boiling, autoclave 121 °C 15 min; cool to about 50 °C and add the supplement |
| Final pH | 7.5 ± 0.2 |
| Storage | Dark, cool and dry place; keep the container tightly closed |
| Shelf life | 3 years |
Applications and practical notes
Yersinia enterocolitica is tested in pork, milk, vegetables and water, for example according to ISO 10273. Typical applications include:
- Pork and pork products
- Raw milk and dairy products
- Vegetables and drinking water
- Veterinary and clinical laboratories
Working with CIN Agar Base: Add the CIN Agar Supplement only after the sterile base has cooled to about 50 °C, and read the plates after about 24 hours at 30 °C.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Shigella and Yersinia methods: ISO 21567 describes the detection of Shigella in food by enrichment in Shigella broth with novobiocin, plating on selective agars such as MacConkey, XLD and Hektoen enteric agar, and biochemical and serological confirmation. ISO 10273 describes the detection of presumptive pathogenic Yersinia enterocolitica by enrichment at 25 °C and plating on CIN agar, followed by tests that separate pathogenic from non-pathogenic strains. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
CIN Agar Base is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include CIN Agar Base, please send us a request. With every delivery of CIN Agar Base you receive the lot number and expiry date, and on request we send the available documentation for CIN Agar Base.
Where CIN Agar Base fits in Shigella and Yersinia testing
- Enrichment – Shigella broth with selective supplement; for Yersinia a peptone-sorbitol-bile type broth
- Selective isolation – Chromogenic Shigella Agar, Desoxycholate Citrate Agar, XLD; for Yersinia CIN agar and Chromogenic Yersinia enterocolitica Agar
- Screening – qPCR or LAMP kits after enrichment
- Confirmation and QC – biochemical identification kits and reference strains
See all Shigella and Yersinia products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| CIN Agar Base | PRO8173 | 500 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
