E. coli O157 · Biochemical identification
E. coli O157 Biochemical Identification Kit – confirm suspect O157 colonies
The E. coli O157 Biochemical Identification Kit confirms suspect E. coli O157:H7 and O157:NM colonies from SMAC or chromogenic O157 agar with a miniaturised biochemical strip and reagents.
- 11-well biochemical strip
- Includes sorbitol and MUG reactions
- Reagents for indole, VP, MR and oxidase
How E. coli O157 Biochemical Identification Kit works
A suspension of a purified colony is dispensed into the strip and incubated. O157 is typically sorbitol negative or delayed and MUG negative, which separates it from other E. coli. Serological confirmation follows with the O157 latex kit.
Typical results
| Result | Read-out |
| E. coli O157 | Sorbitol −/delayed, MR +, VP −, oxidase −, MUG − |
Technical data
| Preparation | Purified colony to 0.5 McFarland; 2 mL into 11-well strip, seal; paraffin over wells 6-8; also TSI (not included) and semi-solid agar; reagents for indole, VP, MR, oxidase |
| Incubation | 36 ± 1 °C, 24 h (cellobiose 24-48 h) |
| Storage | 2-8 °C |
Applications and practical notes
E. coli O157 and other Shiga toxin-producing E. coli (STEC) are tested in meat, raw milk products, sprouts and vegetables, for example according to ISO 16654 and FDA BAM Chapter 4. Typical applications include:
- Raw and minced meat, especially beef
- Raw milk and raw-milk cheese
- Sprouts, leafy greens and other fresh produce
- Outbreak investigations and environmental samples
Working with E. coli O157 Biochemical Identification Kit: Start from a pure colony and combine the result with TSI and the O157 latex test.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Salmonella culture method: the reference method for Salmonella in the food chain, ISO 6579-1, has four steps: non-selective pre-enrichment, selective enrichment in two broths, plating on two selective agars and confirmation of typical colonies. The FDA BAM Chapter 5 follows the same principle with media adapted to different food types. Rapid tests and identification kits shorten the time to result, but positive screening results are usually confirmed by the culture method. COLO.Science offers products for every step, so that one supplier covers the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
E. coli O157 Biochemical Identification Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include E. coli O157 Biochemical Identification Kit, please send us a request. With every delivery of E. coli O157 Biochemical Identification Kit you receive the lot number and expiry date, and on request we send the available documentation for E. coli O157 Biochemical Identification Kit.
Where E. coli O157 Biochemical Identification Kit fits in E. coli O157 testing
- Presumptive test – Lauryl Sulfate Tryptose Broth (MPN) or VRBL / chromogenic agar (colony count)
- Confirmation – BGLB Broth for coliforms; EC or EC-MUG Broth at 44.5 °C for E. coli
- Isolation – EMB, MacConkey, TBX or chromogenic E. coli agar
- Identification – IMViC kit; for O157 and STEC: SMAC or chromogenic O157 agar, latex and PCR
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| E. coli O157 Biochemical Identification Kit | PRO7889 | 10 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
