Staphylococcus aureus · Real-time PCR
Staphylococcus aureus qPCR Detection Kit – real-time PCR with fluorescent probe
The Staphylococcus aureus qPCR Detection Kit detects S. aureus DNA by real-time PCR with a fluorescent probe after enrichment. The kit contains ready-to-use reaction mix, lysis solution and controls.
- Real-time PCR, FAM channel
- Detection limit about 100 CFU per test
- Result within hours after enrichment
How Staphylococcus aureus qPCR Detection Kit works
After enrichment, cells are lysed at 99 °C and the DNA is amplified with S. aureus-specific primers and probe. The Ct value shows whether the target is present: low Ct values indicate positive samples, high or absent Ct values negative ones.
Typical results
| Result | Read-out |
| Positive | Ct ≤ 35 |
| Retest | Ct 35–37 |
| Negative | Ct ≥ 37 or no Ct |
Technical data
| Preparation | Ready-to-use reagents; sample lysis 99 °C 10 min |
| Incubation | Enrichment 36 ± 1 °C, 18-24 h; PCR 95 °C 30 s + 40 cycles (95 °C 5 s / 60 °C 30 s) |
| Storage | -20 °C, protected from light; avoid repeated freeze-thaw |
| Shelf life | 12 months |
Applications and practical notes
Rapid tests for Staphylococcus aureus give a screening result faster than the culture method of ISO 6888-1. Typical applications include:
- Screening of food samples for S. aureus
- Hygiene checks in production, catering and retail
- Laboratories that need results within a working day
- Follow-up of positive samples by the culture method
Working with Staphylococcus aureus qPCR Detection Kit: Separate the areas for sample preparation and PCR, and run a positive and negative control in every run.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Staphylococcus aureus methods: coagulase-positive staphylococci in the food chain are counted on Baird-Parker Agar according to ISO 6888-1, and typical colonies are confirmed with the coagulase test. For low numbers, ISO 6888-3 uses an MPN technique with Giolitti-Cantoni Broth, and the FDA BAM Chapter 12 describes direct plating and an MPN method with Tryptic Soy Broth containing 10 % NaCl. In pharmaceutical microbiology, the test for S. aureus in EP 2.6.13 and USP <62> uses Mannitol Salt Agar. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Staphylococcus aureus qPCR Detection Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Staphylococcus aureus qPCR Detection Kit, please send us a request. With every delivery of Staphylococcus aureus qPCR Detection Kit you receive the lot number and expiry date, and on request we send the available documentation for Staphylococcus aureus qPCR Detection Kit.
Where Staphylococcus aureus qPCR Detection Kit fits in Staphylococcus aureus testing
- Colony count – Baird-Parker Agar with Egg Yolk Tellurite Emulsion (ISO 6888-1); alternatives: chromogenic S. aureus agar or Vogel-Johnson Agar
- Low numbers (MPN) – Giolitti-Cantoni Broth (ISO 6888-3) or Tryptic Soy Broth with 10 % NaCl (FDA BAM), then plating on Baird-Parker Agar
- Confirmation – coagulase test with Rabbit Plasma, latex kit or DNase Agar
- Pharmaceutical products – Mannitol Salt Agar for the S. aureus test of EP 2.6.13 / USP <62>; QC strains for media testing
See the complete Staphylococcus set in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Staphylococcus aureus qPCR Detection Kit | PRO7391 | 48 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
