Vibrio · QC reference strain
Vibrio cholerae non-O1 QC Strain – reference strain for Vibrio media
This non-O1 Vibrio cholerae strain is a qualitative reference strain for growth-promotion testing of TCBS Agar, alkaline peptone water and chromogenic Vibrio media, and for positive controls in routine methods.
- Non-O1 V. cholerae, lyophilised
- For growth-promotion and controls
- Recovery fluid included
How Vibrio cholerae non-O1 QC Strain is used
The lyophilised culture is rehydrated with 1 mL of the supplied fluid, spread on a non-selective saline agar and subcultured. On TCBS Agar it forms yellow colonies, on chromogenic Vibrio cholerae Agar green to blue-green colonies.
Technical data
| Preparation | Add 1.0 mL of the supplied recovery fluid, vortex and spread 0.1 mL on a non-selective saline agar or into broth |
| Incubation | 36 ± 1 °C, 24 h |
| Storage | As stated on the label; use the rehydrated suspension within 8 hours (2–8 °C) |
Applications and practical notes
Rapid tests shorten the detection of Vibrio after enrichment, while positive results are confirmed by the culture method of ISO 21872-1. Typical applications include:
- Screening of seafood batches
- Aquaculture monitoring
- Laboratories that need results within a working day
- Confirmation of suspect colonies
Working with Vibrio cholerae non-O1 QC Strain: Handle the strain in a microbiology laboratory with the appropriate biosafety level and subculture it no more than five times from the original vial.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Vibrio method: ISO 21872-1 describes the detection of potentially enteropathogenic Vibrio parahaemolyticus, V. cholerae and V. vulnificus in seafood and other foods. Samples are enriched twice in Alkaline Saline Peptone Water, plated on TCBS Agar and a second selective medium, and typical colonies are purified on saline agar and confirmed biochemically or by PCR. The FDA BAM Chapter 9 follows a similar scheme. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Vibrio cholerae non-O1 QC Strain is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Vibrio cholerae non-O1 QC Strain, please send us a request. With every delivery of Vibrio cholerae non-O1 QC Strain you receive the lot number and expiry date, and on request we send the available documentation for Vibrio cholerae non-O1 QC Strain.
Where Vibrio cholerae non-O1 QC Strain fits in Vibrio testing
- Enrichment – Alkaline Saline Peptone Water (ASPW), 37 °C and 41.5 °C
- Isolation – TCBS Agar plus a second medium: chromogenic Vibrio agar, mCPC or CC agar
- Purification – non-selective saline agar such as T1N1 Agar
- Confirmation – oxidase, biochemical identification, PCR or LAMP
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Vibrio cholerae non-O1 QC Strain | PRO7860 | 1 vial |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
