Raw material · Selective agent
Ox Bile Salts – raw material for culture media
Ox Bile Salts – bile salts are selective agents of enteric media. They inhibit Gram-positive bacteria while bile-tolerant Gram-negative bacteria such as Enterobacteriaceae can grow.
- Selective agent for enteric media
- Inhibits Gram-positive bacteria
- 100 g pack
How Ox Bile Salts works
Bile salts act as detergents on the cell envelope of Gram-positive bacteria. Enteric bacteria are adapted to bile in the intestine and tolerate the concentrations used in media.
Applications and practical notes
Raw materials are used to prepare culture media in-house, to modify standard formulations and in fermentation and bioprocess media. Typical applications include:
- Preparation of culture media from single components
- Fermentation and bioprocess media
- Research and method development
- Production of media in larger volumes
Working with Ox Bile Salts: Weigh the salts carefully, because the selectivity of the medium depends on the exact concentration.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About culture media raw materials: the performance of a culture medium depends on the quality and consistency of its ingredients – peptones and extracts provide nitrogen, vitamins and growth factors, agar the gel, and bile salts or deoxycholate the selectivity. Laboratories that prepare their own media, and producers of media and fermentation broths, verify each new lot of raw material with reference strains according to ISO 11133. COLO.Science supplies raw materials in laboratory and bulk packs together with ready-made dehydrated media.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Ox Bile Salts is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Ox Bile Salts, please send us a request. With every delivery of Ox Bile Salts you receive the lot number and expiry date, and on request we send the available documentation for Ox Bile Salts.
Where Ox Bile Salts fits in culture media preparation
- Gelling agents – bacteriological, reagent and technical grade agar
- Nitrogen sources – peptones, tryptone, soy peptone, proteose peptone, polypeptone and casein hydrolysates
- Growth factors – yeast extract, beef extract and malt extract
- Selective and special components – bile salts, sodium deoxycholate, soy lecithin, casein and lactose
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Ox Bile Salts | PRO7766 | 100 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
