Pseudomonas aeruginosa · QC reference strain
Pseudomonas aeruginosa QC Strain – CMCC(B) 10104 reference strain
Pseudomonas aeruginosa CMCC(B) 10104 is a qualitative reference strain for growth-promotion testing of CN agar, Cetrimide Agar, chromogenic Pseudomonas media and general media, and for positive controls in routine methods.
- Lyophilised, single use
- For growth-promotion and controls
- Recovery fluid included
How Pseudomonas aeruginosa QC Strain is used
The lyophilised culture is rehydrated with 1 mL of the supplied fluid, spread on a non-selective agar and subcultured. Purity, pigment and typical colony appearance are checked before use.
Technical data
| Preparation | Add 1.0 mL of the supplied recovery fluid, vortex and spread 0.1 mL on a non-selective plate or into broth |
| Incubation | 36 ± 1 °C, 24–48 h |
| Storage | As stated on the label; use the rehydrated suspension within 8 hours (2–8 °C) |
Applications and practical notes
Pseudomonas aeruginosa reference strains are used for growth-promotion tests of selective media, in pharmacopoeial microbiological tests and for media testing according to ISO 11133. Typical applications include:
- Growth-promotion testing of CN, Cetrimide and chromogenic media
- Pharmacopoeial tests for specified micro-organisms
- Positive controls for P. aeruginosa methods
- Method verification and proficiency testing
Working with Pseudomonas aeruginosa QC Strain: Subculture the strain no more than five times from the original vial and record the passage number.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Pseudomonas methods: ISO 16266 describes the detection and enumeration of Pseudomonas aeruginosa in water by membrane filtration on CN agar, where blue-green colonies that produce pyocyanin are counted as confirmed and other typical colonies are confirmed with further tests. ISO 13720 gives the count of presumptive Pseudomonas spp. in meat and meat products on CFC agar at 25 °C. Pharmacopoeial tests for P. aeruginosa use Cetrimide Agar. COLO.Science offers media, supplements, rapid tests and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Pseudomonas aeruginosa QC Strain is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Pseudomonas aeruginosa QC Strain, please send us a request. With every delivery of Pseudomonas aeruginosa QC Strain you receive the lot number and expiry date, and on request we send the available documentation for Pseudomonas aeruginosa QC Strain.
Where Pseudomonas aeruginosa QC Strain fits in Pseudomonas testing
- Water – P. aeruginosa – membrane filtration on CN agar (Pseudomonas Agar Base with CN supplement), 36 °C, 44 h
- Food – Pseudomonas spp. – CFC agar (Pseudomonas Agar Base with CFC supplement), 25 °C, 44 h
- Isolation and screening – Pseudomonas Chromogenic Medium and Cetrimide Agar
- Confirmation and QC – qPCR or LAMP and P. aeruginosa reference strains
See all Pseudomonas products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Pseudomonas aeruginosa QC Strain CMCC(B) 10104 | PRO7848 | 1 vial |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
