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Culture media

COLO.Science · Microbiology · Culture Media

Culture media for food, water and pharmaceutical microbiology

Dehydrated and granulated culture media, supplements, ready-to-use count plates, confirmation tests and QC strains – organised by method step, so you can order everything a standard method needs from one supplier. Each product shows the standards it is used with: ISO, USA FDA, EP and USP.

Salmonella detection – the complete culture method

Every product for the classic Salmonella method, in the order of the method steps. Click a step to see all products for it in the catalogue.

Plating media for Salmonella

Selective agars for the isolation step. Most are also available granulated (250 g) – low dust and fast dissolution.

Listeria detection

Enrichment in Half-Fraser and Fraser Broth, plating on a chromogenic agar of the Ottaviani-Agosti type plus PALCAM or Oxford Agar, and confirmation – as in ISO 11290-1. 30 products including supplements, rapid tests and the reference strain.

Coliforms and E. coli

MPN broths, plating and chromogenic agars, water-testing media, rapid test papers, the IMViC identification kit and E. coli reference strains – for methods such as ISO 4831, ISO 4832, ISO 16649 and FDA BAM Chapter 4. 50 products.

E. coli O157 and STEC

Enrichment broths mTSB, mTSB+n and mEC+n with novobiocin, Sorbitol MacConkey and chromogenic agars with supplements, qPCR, LAMP and multiplex PCR kits, biochemical identification, latex and the O157 reference strain. 18 products.

Staphylococcus aureus

Baird-Parker Agar with Egg Yolk Tellurite Emulsion, chromogenic and selective agars, MPN enrichment broths, rabbit plasma for the coagulase test, rapid tests, latex kit and reference strains – for methods such as ISO 6888-1, ISO 6888-3, FDA BAM Chapter 12 and EP 2.6.13 / USP <62>. 27 products.

Yeasts and moulds

DRBC and DG18 Agar for food and feed, rose bengal and chloramphenicol media, Sabouraud media for pharmaceutical tests, media for the cultivation and identification of fungi and Candida, fermentation media and fungal reference strains – for methods such as ISO 21527-1, ISO 21527-2, FDA BAM Chapter 18 and EP 2.6.12 / USP <61>. 35 products.

General-purpose media

Nutrient, BHI and Cooked Meat media, peptone water and diluents, Columbia, blood, chocolate, GC and Bordet-Gengou bases with supplements, Mueller Hinton media for susceptibility testing (EUCAST, CLSI), CLED and chromogenic UTI agar, urease test media, LB, Terrific Broth and BG-11 for molecular biology. 34 products.

Pharmacopoeial media

Tryptic Soy Agar and Broth, Fluid Thioglycollate Medium, Buffered Sodium Chloride-Peptone Solution and neutralising media, and selective media for specified micro-organisms – for microbial enumeration, tests for specified micro-organisms and the sterility test according to Ph. Eur. 2.6.12, 2.6.13 and 2.6.1 and USP <61>, <62> and <71>, and for cosmetics testing.

Cronobacter

Modified Lauryl Sulfate Tryptose Broth with supplement, Cronobacter screening broth supplement, chromogenic Cronobacter agar, detection plate, qPCR, LAMP and duplex PCR kits and biochemical identification for Cronobacter spp. in infant formula and dry foods (ISO 22964).

Bacillus cereus

MYP Agar (powder and granulated) with polymyxin B and egg yolk supplements, chromogenic Bacillus cereus agar, protein crystal stain, biochemical identification and QC strains for presumptive Bacillus cereus (ISO 7932).

Vibrio

Alkaline saline peptone water, TCBS Agar, chromogenic Vibrio agars, mCPC and CC supplements, T1N1 Agar, qPCR and LAMP kits, biochemical identification and a QC strain for Vibrio parahaemolyticus, V. cholerae, V. vulnificus and V. alginolyticus (ISO 21872-1).

Clostridium

TSC Agar with D-cycloserine, Iron Sulphite Agar and supplements, C. perfringens qPCR and LAMP kits, C. botulinum toxin gene PCR kits, C. difficile latex and C. sporogenes QC strains (ISO 7937, ISO 15213-1).

Pseudomonas

Pseudomonas Agar Base with CN, nalidixic acid and CFC supplements, Pseudomonas Chromogenic Medium, P. aeruginosa qPCR and LAMP kits and QC strains (ISO 16266, ISO 13720). Cetrimide Agar is listed under pharmacopoeial media.

Enterococcus and Streptococcus

Slanetz and Bartley Agar, Bile Esculin Azide Agar and Bile Esculin Agar for intestinal enterococci (ISO 7899-2), Blood Agar Base and Mitis Salivarius Agar with potassium tellurite for streptococci, E. faecalis qPCR and LAMP kits and Lancefield latex kits.

Lactic acid bacteria

MRS Agar and Broth, MRS Agar ISO formulation, Modified MRS Supplement for bifidobacteria, M17 Agar and Broth for lactic streptococci, and Raka-Ray Agar with supplement for beer-spoiling lactic acid bacteria (ISO 15214).

Total viable count

Plate Count Agar (powder and granulated), Milk Plate Count Agar, Tryptone Glucose Extract Agar and granulated R2A Agar for food, feed, milk and water (ISO 4833-1, ISO 6730).

Shigella and Yersinia

Chromogenic Shigella Agar with supplement, Shigella enrichment supplement, Desoxycholate Citrate Agar, Chromogenic Yersinia enterocolitica Agar and CIN supplement, qPCR and LAMP kits, identification kits and QC strains (ISO 21567, ISO 10273).

Campylobacter

Bolton and Preston broth supplements, mCCD, Skirrow and Karmali agar supplements, Skirrow and Campylobacter agar bases and a latex kit for thermotolerant Campylobacter (ISO 10272-1).

Legionella

GVPC and BCYE agar bases with supplements, BCYE without cysteine for confirmation, Legionella latex kit and L. pneumophila qPCR and LAMP kits (ISO 11731).

Burkholderia and Brucella

Burkholderia cepacia selective agar (BCSA) and PCFA supplements, Burkholderia gladioli qPCR and LAMP kits, and Brucella qPCR and LAMP kits.

Enterobacteriaceae

Enterobacteria Enrichment Broth Mossel granulated, Kligler Iron Agar, Simmons Citrate Agar and a biochemical identification kit for Enterobacteriaceae (ISO 21528).

Reagents and stains

Gram and spore stains, catalase and oxidase tests, biochemical reagents (indole, methyl red, Voges-Proskauer, nitrate, deaminase, ninhydrin, Nessler, Benedict) and media additives (glycerol, liquid paraffin, TTC).

General QC reference strains

Qualitative and quantitative reference strains for growth promotion, selectivity and biochemical controls according to ISO 11133: Bacillus, Enterobacter, Micrococcus, Proteus and Serratia.

Raw materials for culture media

Agar, peptones, tryptone, soy peptone, casein hydrolysates, yeast, beef and malt extracts, bile salts, sodium deoxycholate, soy lecithin and lactose – in laboratory and bulk packs for preparing culture and fermentation media.

Count plates

Ready-to-use plates for hygiene indicators and spoilage organisms – a fast alternative to pour plates. 11 products.

Products by method step

Step Products Standards Catalogue
Pre-enrichment BPW, Lactose Broth ISO · FDA · EP View 3
Selective enrichment RV, RVS, MKTTn, Tetrathionate, Selenite, MSRV ISO · FDA · EP · USP View 14
Plating XLD, Chromogenic, Hektoen, BSA, BGA, SS, MLCB, XLT-4 ISO · FDA · EP · USP View 12
Confirmation TSI, LIA, Urea, confirmation plate, ID and latex kits ISO · FDA View 6
Rapid tests Detection plate, isothermal kit, qPCR kit – View 3
QC strains Qualitative and quantitative Salmonella strains – View 9
Count plates Total count, coliforms, E. coli, Enterobacteriaceae, yeasts and moulds, LAB, B. cereus – View 11
Listeria Half-Fraser, Fraser, BLEB, chromogenic, PALCAM, Oxford, TSYEA, kits ISO · FDA View 30
Coliforms and E. coli LST, BGLB, EC, VRBL, MacConkey, EMB, TBX, chromogenic, water media, O157/STEC, test papers ISO · FDA View 68
Staphylococcus aureus Baird-Parker, egg yolk tellurite, MSA, Vogel-Johnson, chromogenic, Giolitti-Cantoni, rabbit plasma, rapid tests, QC strains ISO · FDA · EP · USP View 28
Yeasts and moulds DRBC, DG18, rose bengal, Sabouraud, PDA, MEA, Czapek-Dox, chromogenic Candida, WL, Lysine Medium, QC strains ISO · FDA · EP · USP View 35
General-purpose media Nutrient, BHI, Columbia, blood, chocolate and GC bases, Mueller Hinton, CLED, chromogenic UTI, LB, Terrific Broth ISO · CLSI · FDA View 37
Pharmacopoeial media TSA, TSB, FTM, diluents and neutralisers, VRBG, EE Broth, Cetrimide, RCM, Columbia, R2A EP · USP · ISO View 26
Cronobacter mLST broth and supplements, chromogenic agar, detection plate, qPCR, LAMP, PCR, identification ISO View 9
Bacillus cereus MYP Agar, chromogenic agar, polymyxin B, egg yolk, crystal stain, identification, QC strains ISO · FDA View 14
Vibrio ASPW, TCBS, chromogenic Vibrio agars, mCPC and CC supplements, qPCR, LAMP, identification ISO · FDA View 20
Clostridium TSC Agar, Iron Sulphite Agar, supplements, qPCR, LAMP, toxin gene PCR, latex, QC strains ISO View 14
Pseudomonas Pseudomonas Agar Base, CN and CFC supplements, chromogenic medium, qPCR, LAMP, QC strains ISO View 12
Enterococcus and Streptococcus Slanetz and Bartley, bile esculin media, blood agar base, Mitis Salivarius, qPCR, LAMP, latex ISO View 11
Lactic acid bacteria MRS, modified MRS supplement, M17, Raka-Ray ISO View 8
Total viable count PCA, Milk Plate Count Agar, TGEA, R2A granulated ISO View 6
Shigella and Yersinia Chromogenic Shigella and Yersinia agars, DCA, supplements, qPCR, LAMP, ID kits, QC strains ISO View 18
Campylobacter Bolton, Preston, mCCD, Skirrow and Karmali supplements, agar bases, latex ISO View 10
Legionella GVPC and BCYE media with supplements, latex, qPCR, LAMP ISO View 7
Burkholderia and Brucella BCSA and PCFA supplements, B. gladioli and Brucella qPCR and LAMP USP View 6
Enterobacteriaceae EE Broth granulated, Kligler Iron Agar, Simmons Citrate Agar, ID kit ISO · EP · USP View 6
Reagents and stains Gram and spore stain, catalase, oxidase, IMViC and other biochemical reagents, media additives ISO 11133 View 18
General QC reference strains Bacillus, Enterobacter, Micrococcus, Proteus, Serratia; quantitative B. subtilis ISO 11133 View 10
Raw materials Agar, peptones, tryptone, extracts, bile salts, lecithin, lactose ISO 11133 View 48

Further products – reagents and stains, general QC strains and accessories – will be added step by step. Need a medium that is not listed? Send us the method – COLO.Science supplies the complete set.

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