LEGEND UV/Vis scanning spectrophotometer measurement across six beam and bandwidth configurations
Every LEGEND model reads absorbance, transmittance, concentration and energy directly on its 10.1″ touch screen and offers photometric, quantitative, spectral scanning, kinetics and DNA/protein modes on the instrument, without a PC. Wavelength accuracy of ±0.3 nm over the entire 190–1100 nm range (±0.1 nm at 656.1 nm), stray light ≤0.02 %T at 220 nm and noise of 0.00005 Abs at 700 nm place the series in the mid-to-upper performance class of laboratory UV/Vis spectrophotometers on COLO.Science.
All six models share the same touch-screen platform, photometric accuracy, data interfaces and standard accessories. They differ in beam configuration, spectral bandwidth, long-term stability and baseline flatness — see the model comparison below.
SELECTION NOTE — LEGEND UV/VIS SCANNING SPECTROPHOTOMETER
LEGEND-100 (single beam) and LEGEND-200 (double beam) are the 2 nm bandwidth base configurations. LEGEND-150 and LEGEND-250 narrow the bandwidth to 1 nm for pharmacopoeia-type methods. LEGEND-150S and LEGEND-250S add selectable 0.5 / 1 / 2 / 4 / 5 nm bandwidth in a larger housing. Double-beam models offer tighter long-term stability (≤±0.0003 A/h) than single-beam models (≤±0.0005 A/h).
Key characteristics
- Single-beam or double-beam optical system, 1200 lines/mm grating, 190–1100 nm.
- Spectral bandwidth 2 nm, 1 nm or selectable 0.5 / 1 / 2 / 4 / 5 nm depending on model.
- 10.1″ TFT colour capacitive touch screen on an ARM processing core; built-in help menu.
- Automatic wavelength setting; scanning fast / medium / slow up to 4000 nm/min.
- USB host / USB drive / RS232 / SD card output; internal storage of methods and results.
- Automatic light-door technology, baseline stability correction, power-off data protection.
Suitable for
- Quality control of raw materials and finished products in industry and pharma.
- Clinical, biochemistry and life-science laboratories (DNA/protein, kinetics).
- Environmental protection and water-quality testing with UV/Vis methods.
- Petrochemical and food laboratories running full-spectrum scanning.
- Education and research laboratories needing 1 nm or variable bandwidth.
Why choose a LEGEND UV/Vis scanning spectrophotometer for routine and research work
The LEGEND UV/Vis scanning spectrophotometer series is built around one touch-screen platform shared by all six models, so a laboratory can standardise training, cuvettes, accessories and data workflow while selecting the beam configuration and bandwidth that fit each method. LEGEND-100 and LEGEND-200 use a 2 nm bandwidth, LEGEND-150 and LEGEND-250 a 1 nm bandwidth, and LEGEND-150S and LEGEND-250S a selectable 0.5–5 nm bandwidth for higher-resolution methods and method development.
Every model measures in %T, Abs, C and E, performs full-band, sub-band and time (kinetic) scanning with peak/valley detection and spectrum arithmetic, and exports results over USB, RS232 or SD card. Each instrument is delivered with the same standard accessory set — the 4-position 10 mm cuvette holder with 2× quartz and 4× glass cuvettes of 1 cm optical path — and can be extended with the professional LEGEND PC software with USB dongle, an automatic 8-cell changer, a built-in thermal printer, Wi-Fi UV lamp control, Peltier or sipper systems, an integrating sphere or a reflectance accessory. This shared platform is what makes the LEGEND UV/Vis scanning spectrophotometer practical for QC, clinical, environmental and research laboratories that need one instrument family instead of six unrelated spectrophotometers.
LEGEND series — model variants, SKU and configuration
SKU: PRO3301
LEGEND-100
- Optical system: Single beam
- Spectral bandwidth: 2 nm
- Stability: ≤±0.0005 A/h at 500 nm
- Baseline flatness: ±0.0002 A
- Instrument size: 590×475×250 mm
SKU: PRO3302
LEGEND-150
- Optical system: Single beam
- Spectral bandwidth: 1 nm
- Stability: ≤±0.0005 A/h at 500 nm
- Baseline flatness: ±0.0002 A
- Instrument size: 590×475×250 mm
SKU: PRO3303
LEGEND-150S
- Optical system: Single beam
- Spectral bandwidth: 0.5 / 1 / 2 / 4 / 5 nm
- Stability: ≤±0.0005 A/h at 500 nm
- Baseline flatness: ±0.0001 A
- Instrument size: 760×560×280 mm
SKU: PRO3304
LEGEND-200
- Optical system: Double beam
- Spectral bandwidth: 2 nm
- Stability: ≤±0.0003 A/h at 500 nm
- Baseline flatness: ±0.0002 A
- Instrument size: 590×475×250 mm
SKU: PRO3305
LEGEND-250
- Optical system: Double beam
- Spectral bandwidth: 1 nm
- Stability: ≤±0.0003 A/h at 500 nm
- Baseline flatness: ±0.0002 A
- Instrument size: 590×475×250 mm
SKU: PRO3306
LEGEND-250S
- Optical system: Double beam
- Spectral bandwidth: 0.5 / 1 / 2 / 4 / 5 nm
- Stability: ≤±0.0003 A/h at 500 nm
- Baseline flatness: ±0.0001 A
- Instrument size: 760×560×280 mm
Every LEGEND UV/Vis scanning spectrophotometer model: 190–1100 nm, wavelength accuracy ±0.3 nm (±0.1 nm at 656.1 nm), photometric accuracy ±0.0025 A at 1.0 Abs, 10.1″ touch screen, USB / RS232 / SD card output. Stability is specified at 500 nm.
Technical specifications — LEGEND UV/Vis scanning spectrophotometer common platform
| Optical system | Single beam (LEGEND-100/150/150S) or double beam (LEGEND-200/250/250S), grating 1200 lines/mm |
| Wavelength range | 190–1100 nm |
| Wavelength accuracy | ±0.3 nm (entire range); ±0.1 nm at 656.1 nm |
| Wavelength repeatability | ≤0.1 nm |
| Photometric accuracy | ±0.0025 A at 1.0 Abs |
| Photometric repeatability | ±0.0002 at 0.5 A; ±0.0006 at 1.0 A; ±0.001 at 2.0 A |
| Photometric range | 0–400 %T, −4 to 4 A, 0–9999 C (0–9999 F) |
| Stray light | ≤0.02 %T at 220 nm |
| Noise | 0.00005 Abs at 700 nm |
| Baseline stability | <0.0003 Abs/h |
| Light source | Deuterium (D2) / tungsten-halogen lamp |
| Detector | Silicon photodiode |
| Display | 10.1″ TFT colour capacitive touch screen, ARM processing core |
| Measuring modes | %T, Abs, C, E; photometric, quantitative, spectral scanning, kinetics, DNA/protein |
| Scanning | Automatic wavelength setting; fast / medium / slow, max 4000 nm/min; full-band, sub-band and time scanning |
| Cuvette holder | 4-position 10 mm manual (standard); 5–100 mm, up to 8 positions, manual or automatic (option) |
| Data output | USB host / USB drive / RS232 / SD card |
| Power supply | AC 220 V ±10 %, 50/60 Hz, 100 W |
| Packing size / weight | 810×660×390 mm, 20 / 33 kg (LEGEND-100/150/200/250); 880×690×520 mm, 28 / 47 kg (LEGEND-150S/250S) |
Final performance of a LEGEND UV/Vis scanning spectrophotometer depends on installation, environmental stability and calibration routine. Final model configuration and accessories are confirmed in the official quotation and in the PDF technical specification.
Operating and measurement functions
Each LEGEND UV/Vis scanning spectrophotometer is operated from its 10.1″ capacitive touch screen: measurement mode, wavelength, calibration curve and scan parameters are set on screen, curves and results are displayed on the instrument, and data can be imported or exported without a PC.
Photometric and quantitative
Direct reading in %T, Abs, C and E; automatic concentration determination via calibration curve with linear regression; single and multi-wavelength measurement.
Spectral scanning and kinetics
Full-band, sub-band and time scanning up to 4000 nm/min; spectrum subtraction and addition, peak/valley detection, peak area and peak overlay; DNA/protein mode.
Data and connectivity
USB host / USB drive / RS232 / SD card; internal storage of methods and results; power-off protection keeps data, spectra, regression equations and correction parameters.
Safety and utilities of the LEGEND UV/Vis scanning spectrophotometer
- Automatic light-door technology — no black body needed, protects the photoelectric detector.
- Baseline stability correction and power-off protection for rapid re-initialisation.
- Optional Wi-Fi UV lamp control switches the deuterium lamp on/off separately from the touch screen or an Android device to extend lamp life.
- Power supply AC 220 V ±10 %, 50/60 Hz, rated power 100 W; use a grounded outlet.
- 21 CFR Part 11–compliant software available as an option for regulated laboratories.
Standard delivery, accessories and options for the LEGEND UV/Vis scanning spectrophotometer
INCLUDED IN STANDARD DELIVERY
Standard cuvette set and accessories
- Spectrophotometer with the 4-position 10 mm cuvette holder fitted
- 2× quartz cuvettes, 1 cm (10 mm) optical path — UV region
- 4× glass cuvettes, 1 cm (10 mm) optical path — visible region
- Power cable with Euro plug, AC 220 V, 50/60 Hz
- Instruction manual in English
OPTION — NOT INCLUDED AS STANDARD
Professional LEGEND PC software with USB dongle
The optional professional LEGEND software moves full instrument control, acquisition, data storage and processing to the PC via the USB communication port:
- Live spectral scanning with on-screen curve display, peak/valley detection, spectrum arithmetic and overlay
- Quantitative analysis with stored calibration curves, kinetics / time scan, multi-wavelength, DNA/protein
- Storage of large data files, report generation and export for further processing
- Licensed by USB hardware dongle — runs on any compatible PC with the dongle connected
- 21 CFR Part 11–compliant version available for regulated laboratories
Optional accessories and consumables
| Accessory | Purpose / notes |
|---|---|
| Professional LEGEND software with USB dongle | Full PC control: scanning, kinetics, quantitative analysis, multi-wavelength measurement, reporting |
| 21 CFR Part 11–compliant software | Regulated environments — audit trail, electronic signatures, user access rights |
| Automatic 8-cell changer | Motorised holder for unattended sequential measurement of up to 8 samples |
| 5 cm / 10 cm cuvette holder, manual or automatic | Long-path cells for low-concentration samples; the sample chamber accepts 5–100 mm optical path |
| Peltier temperature-control system | Electronic thermostatting of the cuvette for temperature-dependent work and enzyme kinetics |
| Sipper (peristaltic) system | Automatic sample aspiration into a flow cell — faster serial measurement with less carry-over |
| Integrating sphere | Measurement of scattering, turbid and solid samples |
| Reflectance accessory | Reflectance measurement on solid samples and surfaces |
| Film / solid sample holder | Holder for films, foils and plate-shaped solid samples |
| Test-tube rack | Direct measurement in round test tubes |
| Cuvettes, 5–100 mm optical path | Quartz and glass cuvettes in 5, 10, 20, 30, 50 and 100 mm path lengths |
| Semi-micro and micro cuvettes | Quartz cells for small sample volumes (DNA/protein, limited-volume samples) |
| Flow cell | Quartz flow cuvette, used together with the sipper system |
| Built-in thermal printer | Direct printout of test results and reports on the instrument, without a PC |
| Wi-Fi UV lamp control | Switch the deuterium lamp on/off separately via Android device or touch screen to extend lamp life |
| Spare deuterium / tungsten-halogen lamp | Replacement lamps for the dual-lamp source |
Accessory availability depends on the model configuration — please state the instrument model (LEGEND-100 / 150 / 150S / 200 / 250 / 250S) when requesting accessories, so that compatibility of the cuvette holder and sample chamber can be confirmed. Instrument calibration and/or issuance of a service/calibration certificate is billed separately, quoted on request.
SPECTROPHOTOMETERS OVERVIEW
Compare the LEGEND series with other COLO.Science spectrophotometers.
SPECTROPHOTOMETERS CATALOGUE
Selection support for bandwidth, beam configuration and accessories.
PDF TECHNICAL SPECIFICATION
Five-page LEGEND data sheet with model table, accessories and contacts.
Applications and operational value of the LEGEND UV/Vis scanning spectrophotometer
Routine QC
Touch-screen operation, stored methods and automatic concentration determination support fast, repeatable QC measurement of raw materials and finished products.
Clinical and biochemistry
DNA/protein and kinetics modes, micro cuvettes and the optional Peltier system cover life-science and clinical-chemistry workflows.
Environmental and petrochemical
190–1100 nm coverage with scanning up to 4000 nm/min and ≤0.02 %T stray light supports UV/Vis water, environmental and petrochemical methods.
Documentation and compliance
USB / RS232 / SD card export, the built-in thermal printer option and the 21 CFR Part 11 software version keep results traceable.
How to select the appropriate LEGEND UV/Vis scanning spectrophotometer model
Choosing the right LEGEND UV/Vis scanning spectrophotometer model starts from the spectral bandwidth the method calls for. Methods that specify 2 nm are covered by LEGEND-100 or LEGEND-200; pharmacopoeia-type methods that require 1 nm point to LEGEND-150 or LEGEND-250; laboratories running several methods with different resolution requirements, or developing methods, benefit from the selectable 0.5 / 1 / 2 / 4 / 5 nm bandwidth of LEGEND-150S and LEGEND-250S.
The second decision is the beam configuration. Single-beam models (LEGEND-100 / 150 / 150S) are the economical choice for routine work; double-beam models (LEGEND-200 / 250 / 250S) compensate lamp drift continuously and offer tighter long-term stability (≤±0.0003 A/h) for long kinetic runs and low-absorbance work. Note that the “S” models come in a larger housing (760×560×280 mm). All six models accept the same standard and optional accessories, so the accessory decision — software, printer, automatic cell changer, long-path cuvettes, calibration — can be made independently of the model choice.
Configuration notice: every LEGEND UV/Vis scanning spectrophotometer can be configured according to the application and accessories (software, printer, holders, etc.). The official PDF technical specification defines the exact delivered configuration, included components and available options. The manufacturer reserves the right to change technical specifications. Final configuration and included items are defined by the official PDF and quotation.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for the LEGEND UV/Vis scanning spectrophotometer series across Europe. B2B prices are quoted net, excluding VAT.
