LEGEND-PMT Double Beam UV/Vis Spectrophotometer, PMT Detector, 190–900 nm

COLO.Science · Spectroscopy & Color · UV/Vis spectrophotometers

LEGEND-PMT double beam UV/Vis spectrophotometer with PMT detector — 190–900 nm

The LEGEND-PMT double beam UV/Vis spectrophotometer combines a reference-beam optical system with a photomultiplier tube (PMT) detector. It covers 190–900 nm, scans at up to 3000 nm/min and is available in three versions — spectral bandwidth 2 nm, 1 nm or selectable 0.5 / 1 / 2 / 4 / 5 nm — for laboratories that need high sensitivity, a stable baseline and reliable readings at high absorbance.

Which UV/Vis spectrophotometer?

Single and split beam — routine Vis and UV/Vis

Split beam · Vis · scanningNOVEL3S325–1100 nm · bandwidth 2 nm · auto 8-cell holder · 8″ touch screenSingle beam · scanningNOVEL4S190–1100 nm · bandwidth 2 nm · 7″ touch screenSingle beam · 4 modelsMAVRICA18190–1100 nm · bandwidth 0.5–5 nm · USB/RS232, Excel exportSingle & double beam · 6 modelsLEGEND190–1100 nm · bandwidth 0.5–5 nm · 10.1″ touch screen

Double beam, variable bandwidth and PMT

Double beam · variable bandwidthNOVEL-900Selectable 0.5 / 1 / 2 / 4 / 5 nm · stray light ≤0.01 %TDouble & split beam · GLPMAVRICA-T11″ touch screen · cloud, Bluetooth, Wi-Fi data outputDouble beam · PMT detector · 3 modelsLEGEND-PMTBandwidth 2 / 1 / 0.5–5 nm · PMT detector · 7″ colour display

Double beam optics and photomultiplier detection

A double beam UV/Vis spectrophotometer measures the sample and the reference at the same time, so lamp drift does not show up in the result: LEGEND-PMT keeps the baseline flat to ±0.001 A and stable to ±0.0005 A per hour. The PMT detector amplifies the light signal inside the tube — noise is only 0.0005 A at 500 nm, stray light ≤0.03 %T at 220 and 360 nm. That combination is what methods in the deep UV, at narrow slits and with strongly absorbing samples need.

Key characteristics

• Double beam UV/Vis spectrophotometer, grating 1200 lines/mm, wavelength range 190–900 nm
• Photomultiplier tube (PMT) detector — noise 0.0005 A at 500 nm, stray light ≤0.03 %T
• Three bandwidth versions: LEGEND-PMT200 2 nm · LEGEND-PMT250 1 nm · LEGEND-PMT250S 0.5 / 1 / 2 / 4 / 5 nm
• Wavelength accuracy ±0.1 nm at 656.1 nm (±0.3 nm over the range), repeatability ≤0.1 nm
• Photometric range −4 to 4 A; accuracy ±0.002 A (0–0.5 A); stability ±0.0005 A/h
• Scanning up to 3000 nm/min, kinetics, quantitation, multi-wavelength and protein / DNA test; 7″ colour display, USB and RS232

Suitable for

• Pharmaceutical laboratories: identification, assay and dissolution testing, methods with a prescribed bandwidth
• Biochemistry and life sciences: nucleic acids and proteins at 260/280 nm, enzyme kinetics
• Research and university laboratories that need full spectra and narrow bandwidths
• Quality control of chemicals, petrochemical and industrial products
• Environmental and water laboratories with UV methods such as nitrate

Why a UV/Vis spectrophotometer with a PMT detector

In a photomultiplier tube, light striking the photocathode releases electrons that are multiplied along a chain of dynodes — the signal is amplified inside the detector, before any electronics. A silicon photodiode has no such gain. The difference shows whenever little light reaches the detector: dense or strongly absorbing samples, the deep UV near 190–230 nm where lamp energy is lowest, and narrow slits of 1 nm or 0.5 nm.

The PMT is optimised for the UV and visible range, which is why LEGEND-PMT covers 190–900 nm. For measurements in the near-infrared up to 1100 nm, choose a model with a silicon photodiode — LEGEND, NOVEL-900 or MAVRICA-T — from the menu above.

Cell holders and sample handling

The large sample compartment of the LEGEND-PMT double beam UV/Vis spectrophotometer accepts cuvettes with path lengths from 5 to 100 mm. A single 10 mm cell holder and six cuvettes are in standard delivery; automatic 8-cell holder, film holders, integrating sphere, reflectance accessory and a Peltier / sipper system are available as options.

Quartz and glass cuvettes2 quartz and 4 glass cuvettes, 10 mm, in standard delivery.
Standard

Automatic 8-cell holderMeasures a series of samples without opening the lid.
Option

Manual 4-cell holder up to 100 mmLong path lengths for low concentrations.
Option

Single cell holder 10 mmOne fixed position for single measurements.
Option

Adjustable XY micro cell holderAligns micro cuvettes for small sample volumes.
Option

Single-hole film holderTransmittance of films, filters and glass.
Option

Manual 4-position film holderFour film samples in one series.
Option

Integrating sphereDiffuse reflectance of solids and powders.
Option

Test-tube rackMeasurement directly in round tubes.
Option

Peltier / sipper systemTemperature control and sample aspiration.
Option

Operation and data

Photometry, wavelength scan, quantitation, multi-wavelength measurement, kinetics and the protein / DNA test are started directly from the 7″ colour display. The wavelength is set automatically; the scanning speed is selectable up to 3000 nm/min.

Up to 1024 MB of test data and working curves stay in the instrument; via USB the results go to a PC or straight into Excel, RS232 is available as well. PC software is in standard delivery, 21 CFR Part 11 compliant software and a built-in thermal printer are options.

Standard delivery

• LEGEND-PMT double beam UV/Vis spectrophotometer (PMT200, PMT250 or PMT250S)
• Single cell holder, 10 mm
• 4 × glass cuvette and 2 × quartz cuvette, 10 mm path length
• PC software, power cable and operation manual

Optional: automatic 8-cell holder, manual 4-cell holder up to 100 mm, micro cell holder, film holders, integrating sphere, reflectance accessory, Peltier / sipper system, test-tube rack, built-in thermal printer and 21 CFR Part 11 software. The delivered configuration for a specific order is the one confirmed in the COLO.Science quotation.

Knowledge base

Spectral bandwidth, stray light, Beer–Lambert law and the choice of cuvettes are explained on COLO.Science — UV-Vis spectrophotometry and colour measurement, our knowledge base; how absorbance fits among other laboratory measurements is described in Measurement in laboratory science. The full data are in the LEGEND-PMT technical specification (PDF).

Selection guide

• Routine visible methods in series: NOVEL3S Vis scanning spectrophotometer — 325–1100 nm, split beam, automatic 8-cell holder.
• Full UV/Vis range at 2 nm with scanning: NOVEL4S — 190–1100 nm, compact single beam, 7″ touch screen.
• Several bandwidths in one single-beam instrument: MAVRICA18 or LEGEND series — bandwidth from 0.5 to 5 nm.
• Long sequences, baseline stability, resolution checks: NOVEL-900 double beam with selectable 0.5–5 nm.
• GLP data management: MAVRICA-T series — 11″ touch screen, cloud and wireless data output.
• Highest sensitivity, deep UV and narrow bandwidths: LEGEND-PMT double beam UV/Vis spectrophotometer with PMT detector — 190–900 nm.

Configuration notice

This page is provided for orientation and product selection. Cell holders, cuvettes, printer, software and other options are confirmed with the official COLO.Science quotation.

Sales and support for international customers

COLO Lab ExpertsPolje ob Sotli 4, SI-3255 Buče, Slovenia
Phone: +386 70 713 437 · Email: sales@colo.si · Web: colo.si
We supply laboratories across Europe directly and through partners. B2B prices are quoted net, excluding VAT.

Need help choosing a spectrophotometer?

Considering the LEGEND-PMT double beam UV/Vis spectrophotometer? Send us your samples, the wavelengths and methods you use and the number of samples per day — we propose the bandwidth version, cell holders and cuvettes and confirm them with the quotation.