Salmonella detection · Step 2 – selective enrichment
RVS Broth – selective enrichment broth for Salmonella
Rappaport-Vassiliadis Medium with Soya (RVS Broth) is a selective enrichment broth for Salmonella from food, feed and environmental samples. It is inoculated from the pre-enrichment culture and used in parallel with a second enrichment broth, usually MKTTn Broth, before plating on selective agars.
- Selective enrichment step of the Salmonella culture method
- Incubation at elevated temperature for higher selectivity
- Dehydrated powder, 500 g bottle · COLO code PRO7567
How RVS Broth works
Magnesium chloride raises the osmotic pressure and malachite green inhibits most accompanying bacteria, while Salmonella tolerates both. The low pH and incubation at elevated temperature increase the selectivity further. Soya peptone supports the growth of Salmonella after the transfer from pre-enrichment.
Technical data
| Preparation | 26.6 g per litre of water; dissolve, dispense and sterilise at 115 °C for 15 min |
| Final pH | 5.2 ± 0.2 |
| Incubation | 42 °C, 18–24 h |
| Storage | Dry, dark place; close the cap tightly after use |
| Shelf life | See the product label |
Applications and practical notes
RVS Broth is the first of the two selective enrichment broths in ISO 6579-1. Typical applications include:
- meat, poultry, eggs and egg products
- milk, dairy products and infant formula
- feed, spices and dry foods
- environmental swabs from the food production area
Working with RVS Broth: A small inoculum, usually 0.1 mL of pre-enrichment culture in 10 mL of RVS Broth, keeps the selective effect strong. Incubation at elevated temperature is essential, so the incubator temperature should be checked regularly.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Salmonella culture method: the reference method for Salmonella in the food chain, ISO 6579-1, has four steps: non-selective pre-enrichment, selective enrichment in two broths, plating on two selective agars and confirmation of typical colonies. The FDA BAM Chapter 5 follows the same principle with media adapted to different food types. Rapid tests and identification kits shorten the time to result, but positive screening results are usually confirmed by the culture method. COLO.Science offers products for every step, so that one supplier covers the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
RVS Broth is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include RVS Broth, please send us a request.
Where RVS Broth fits in the Salmonella workflow
- Pre-enrichment – Buffered Peptone Water or Lactose Broth
- Selective enrichment – RVS Broth and MKTTn Broth
- Selective isolation – XLD Agar plus a second selective or chromogenic agar
- Confirmation – TSI, Lysine Iron Agar, Urea Agar, latex and biochemical identification
See the complete Salmonella set in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| RVS Broth, dehydrated | PRO7567 | 500 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
