Salmonella detection · Step 2 – selective enrichment
Selenite Enrichment Medium – selective enrichment of Salmonella
Selenite Enrichment Medium is a selective enrichment broth for Salmonella from food and other samples with a high accompanying flora. It is prepared without autoclaving and inoculated from the pre-enrichment culture or directly with the sample.
- Selective enrichment of Salmonella
- Prepared without autoclaving
- Dehydrated powder, 500 g bottle · COLO code PRO7568
How Selenite Enrichment Medium works
Sodium selenite inhibits coliforms and enterococci, while Salmonella continues to grow. Lactose fermentation by accompanying bacteria lowers the pH, which keeps the selenite selective during incubation.
Applications and practical notes
Selenite Enrichment Medium is used for the selective enrichment of Salmonella from foods and other samples. Typical applications include:
- dried and processed foods
- eggs and egg products
- dairy products
- environmental samples
Working with Selenite Enrichment Medium: Do not autoclave the medium and avoid overheating, which can produce a red precipitate of selenium; such medium must be discarded. Sodium selenite is toxic, so the powder should be handled with gloves.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Salmonella culture method: the reference method for Salmonella in the food chain, ISO 6579-1, has four steps: non-selective pre-enrichment, selective enrichment in two broths, plating on two selective agars and confirmation of typical colonies. The FDA BAM Chapter 5 follows the same principle with media adapted to different food types. Rapid tests and identification kits shorten the time to result, but positive screening results are usually confirmed by the culture method. COLO.Science offers products for every step, so that one supplier covers the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Selenite Enrichment Medium is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Selenite Enrichment Medium, please send us a request.
Where Selenite Enrichment Medium fits in the Salmonella workflow
- Pre-enrichment – Buffered Peptone Water or Lactose Broth
- Selective enrichment – RV or RVS Broth and a selenite or tetrathionate broth
- Selective isolation – XLD Agar plus a second selective or chromogenic agar
- Confirmation – TSI, Lysine Iron Agar, Urea Agar, latex and biochemical identification
See the complete Salmonella set in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Selenite Enrichment Medium, dehydrated | PRO7568 | 500 g |
| Selenite Cystine Broth, dehydrated | PRO7436 | 500 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
