Listeria · environmental monitoring
Environmental Listeria Detection Plate – ready-to-use plate for Listeria in the production environment
The Environmental Listeria Detection Plate is a ready-to-use dry film plate for the detection and count of Listeria in environmental samples such as surface swabs and sponges from food production areas. Listeria forms blue-green colonies that are easy to recognise.
- Ready to use, no media preparation
- Listeria: blue-green colonies
- Pack of 20 plates · COLO code PRO7374
How the Environmental Listeria Detection Plate works
The plate contains selective nutrients, a cold-water-soluble gelling agent and a chromogenic indicator. After a short enrichment of the swab, the sample is applied to the plate; during incubation Listeria species, including L. monocytogenes, L. innocua and L. welshimeri, form blue-green colonies.
Reading the plate
| Colonies | Meaning |
| Blue-green colonies | Listeria spp. |
Typical colonies can be confirmed with Listeria identification tests when species identification is needed.
Technical data
| Enrichment | 30 ± 1 °C, 24 ± 2 h |
| Incubation | 36 ± 1 °C, 24 ± 2 h, face up |
| Countable range | 30–300 CFU per plate |
| Storage | 2–8 °C, sealed; after opening reseal the bag and use within 4 weeks |
| Shelf life | 18 months |
Applications and practical notes
The Environmental Listeria Detection Plate is used in environmental monitoring programmes for Listeria, which complement product testing according to ISO 11290-1. Typical applications include:
- surface swabs and sponges from production lines
- drains, floors and conveyor belts
- cold rooms and packing areas
- verification of cleaning and disinfection
Working with Environmental Listeria Detection Plate: Sample the same points at regular intervals and record the results over time, so that trends and recurring positive spots can be recognised. Incubate the plates face up and do not stack too many.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Listeria culture method: the reference method for Listeria monocytogenes in the food chain, ISO 11290-1, uses primary enrichment in Half-Fraser Broth, secondary enrichment in Fraser Broth, plating on a chromogenic agar of the Ottaviani-Agosti type and a second selective agar, and confirmation of typical colonies. The FDA BAM Chapter 10 uses Buffered Listeria Enrichment Broth instead. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Environmental Listeria Detection Plate is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Environmental Listeria Detection Plate, please send us a request. With every delivery of Environmental Listeria Detection Plate you receive the lot number and expiry date, and on request we send the available documentation for Environmental Listeria Detection Plate.
Where the Environmental Listeria Detection Plate fits in the workflow
- Enrichment – Half-Fraser Broth or another Listeria enrichment
- Rapid screening – detection plate, LAMP or real-time PCR
- Confirmation – chromogenic Listeria agar, TSYEA, identification kit and latex
- Report – negative results reported quickly; positives confirmed
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Environmental Listeria Detection Plate | PRO7374 | 20 plates |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
