Listeria detection · media supplement
FB2 Supplement – for secondary enrichment
Selective supplement for preparing the secondary enrichment broth (FB2) from Fraser Broth Base. One vial completes 100 mL.
- Selective supplement for Fraser Broth Base
- Lyophilised, dissolved before use
- 10 vials per box · COLO code PRO7677
How the FB2 Supplement is used
The lyophilised supplement contains nalidixic acid and acriflavine at the concentration used for secondary enrichment. It is dissolved in sterile saline and added to the cooled base.
Technical data
| Composition per vial | nalidixic acid 2.0 mg, acriflavine hydrochloride 2.5 mg |
| Use | Dissolve in 1 mL sterile saline and add to 100 mL of Fraser Broth Base |
| Storage | 2–8 °C |
| Shelf life | 2 years |
Applications and practical notes
The FB2 supplement is used to prepare the secondary enrichment broth for Listeria, as in ISO 11290-1. Typical applications include:
- ready-to-eat foods and delicatessen
- milk and dairy products
- fish and seafood
- environmental samples from food production
Working with FB2 Supplement: Dissolve the vial completely, add it only to the cooled sterile base and mix gently but thoroughly before dispensing or pouring. Keep unopened vials refrigerated.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Salmonella culture method: the reference method for Salmonella in the food chain, ISO 6579-1, has four steps: non-selective pre-enrichment, selective enrichment in two broths, plating on two selective agars and confirmation of typical colonies. The FDA BAM Chapter 5 follows the same principle with media adapted to different food types. Rapid tests and identification kits shorten the time to result, but positive screening results are usually confirmed by the culture method. COLO.Science offers products for every step, so that one supplier covers the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
FB2 Supplement is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include FB2 Supplement, please send us a request. With every delivery of FB2 Supplement you receive the lot number and expiry date, and on request we send the available documentation for FB2 Supplement.
Where FB2 fits in the Listeria workflow
- Primary enrichment – Half-Fraser Broth
- Secondary enrichment – Fraser Broth
- Selective isolation – chromogenic Listeria agar (Ottaviani-Agosti type) plus PALCAM or Oxford Agar
- Confirmation – subculture on TSYEA, biochemical identification and latex
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Fraser Broth (FB2) Supplement | PRO7677 | 10 vials |
| Fraser Broth Base, dehydrated | PRO7446 | 500 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
