Pharmaceutical microbiology · test for Salmonella
Brilliant Green Phenol Red Agar – selective agar for Salmonella
Brilliant Green Phenol Red Agar is a selective plating medium for Salmonella, used in the European Pharmacopoeia test for specified microorganisms in non-sterile products and in food microbiology.
- Salmonella: red colonies with a red zone
- Used in EP methods
- Dehydrated powder, 500 g bottle · COLO code PRO7477
How Brilliant Green Phenol Red Agar works
Brilliant green inhibits Gram-positive bacteria and most coliforms. Bacteria that ferment lactose or sucrose turn phenol red yellow; Salmonella does not ferment these sugars and forms red colonies on a red background.
Typical colony appearance
| Organism | Colonies |
| Salmonella | red, with red zone |
| Lactose / sucrose fermenters | yellow, with yellow zone |
Applications and practical notes
Brilliant Green Phenol Red Agar is used in the European Pharmacopoeia test for Salmonella and in food microbiology. Typical applications include:
- non-sterile pharmaceutical products and raw materials
- herbal products and dietary supplements
- cosmetics and their raw materials
- food and feed samples
Working with Brilliant Green Phenol Red Agar: Do not autoclave the medium and avoid overheating. Typical red colonies are confirmed by identification tests according to the method used.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Salmonella culture method: the reference method for Salmonella in the food chain, ISO 6579-1, has four steps: non-selective pre-enrichment, selective enrichment in two broths, plating on two selective agars and confirmation of typical colonies. The FDA BAM Chapter 5 follows the same principle with media adapted to different food types. Rapid tests and identification kits shorten the time to result, but positive screening results are usually confirmed by the culture method. COLO.Science offers products for every step, so that one supplier covers the whole procedure.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Brilliant Green Phenol Red Agar is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Brilliant Green Phenol Red Agar, please send us a request.
Where Brilliant Green Phenol Red Agar fits in the test for Salmonella
- Enrichment – sample in soya-casein digest broth
- Selective enrichment – Rappaport-Vassiliadis Salmonella Enrichment Broth
- Selective isolation – XLD Agar or another selective agar
- Identification – suspect colonies confirmed by identification tests
See the complete Salmonella set in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Brilliant Green Phenol Red Agar, dehydrated | PRO7477 | 500 g |
| Brilliant Green Agar, dehydrated | PRO7482 | 500 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
