COLO.SCIENCE — CHAMBER-BASED MICROSCOPY WORKFLOW
Fixed-depth quartz glass counting chamber for concentration and motility observation
MAKLER100 supports a direct chamber-based workflow for sperm concentration counting and motility observation under a transmitted-light biological microscope. The fixed 10 µm chamber depth and known 1 mm² grid area help the laboratory work with a defined optical volume and a repeatable counting field when the chamber is used according to local SOP.
For a complete workflow, COLO.Science recommends pairing the MAKLER100 chamber with the OPTIC900TL trinocular LED microscope and a compatible microscope camera. The microscope provides brightfield transmitted illumination, plan optics, mechanical stage movement and a trinocular photo port for documentation, training and QC image review.
Key chamber characteristics
- Model: MAKLER100.
- Product type: sperm counting chamber / optical glass counting chamber.
- Optical path: quartz glass.
- Chamber depth: 10 µm.
- Depth definition: three built-in ruby spacers.
- Counting grid: 1 mm².
- Grid subdivision: 100 squares.
- Square size: 0.1 × 0.1 mm.
- Specimen type: undiluted specimen, according to laboratory SOP.
- Storage: supplied with carrying case.
Workflow support
- Rapid chamber-based sperm concentration workflow.
- Motility observation under brightfield transmitted light.
- Defined optical volume for routine counting procedures.
- Visual grid-based scanning and field selection.
- Camera documentation for training, QC review and reporting support.
- Recommended set with OPTIC900TL trinocular biological microscope and compatible camera.
MAKLER100 — SPERM COUNTING CHAMBER
Sperm counting chamber for microscope-based concentration and motility workflow support
The MAKLER100 sperm counting chamber is designed for laboratories that need a simple optical chamber for rapid concentration counting and motility observation. Quartz glass, a fixed 10 µm chamber height and a 1 mm² grid divided into 100 equal squares support repeatable microscopic observation when the loading method, timing, magnification and counting pattern are controlled.
The recommended microscope platform is an upright biological microscope with transmitted brightfield illumination. The OPTIC900TL trinocular LED microscope is suitable for this type of workflow because it offers 40x–1000x magnification, infinity plan achromatic optics, Koehler LED illumination, mechanical stage control and a trinocular port for camera documentation.
This page describes equipment and workflow support. Clinical interpretation, diagnostic reporting, reference limits, acceptance criteria, sample handling and cleaning procedure must follow the laboratory’s validated SOP, current professional guidance and trained operator practice.
MAKLER100 chamber, carrying case and OPTIC900TL microscope
MAKLER100 chamber with quartz glass optical path, fixed 10 µm chamber depth and 1 mm² grid for microscope-based counting workflow.
The chamber is supplied with a carrying case for storage and protection of the optical glass surfaces between uses.
OPTIC900TL trinocular LED microscope supports brightfield observation, mechanical stage scanning and camera documentation for MAKLER100 workflows.
MAKLER100 — chamber and recommended microscope set
MAKLER100 chamber
Product type: sperm counting chamber
Optical material: quartz glass
Chamber depth: 10 µm
Depth control: three ruby spacers
Counting grid: 1 mm²
Grid subdivision: 100 squares
Square size: 0.1 × 0.1 mm
Specimen: undiluted specimen, according to SOP
Storage: carrying case
Recommended set
Chamber: MAKLER100
Microscope: OPTIC900TL trinocular biological microscope
Illumination: 5W LED Koehler transmitted light
Optics: infinity plan achromatic
Magnification: 40x–1000x with standard optics
Head: trinocular Siedentopf, camera port
Camera: compatible microscope camera / C-mount adapter
Use: counting, motility observation, documentation and training support
Selection note: confirm the intended semen-analysis workflow, microscope configuration, camera/software requirement, adapter, documentation requirement, cleaning procedure and laboratory SOP before ordering.
TECHNICAL SPECIFICATIONS — MAKLER100 SPERM COUNTING CHAMBER
| Product model | MAKLER100 |
| Product type | Sperm counting chamber / optical glass counting chamber |
| Application | Rapid sperm count, concentration workflow, motility observation and semen-analysis workflow support |
| Optical path | Quartz glass |
| Chamber spacing | 10 µm, provided by three built-in ruby spacers |
| Counting grid | 1 mm² grid |
| Grid subdivision | 100 squares, each 0.1 × 0.1 mm |
| Specimen | Undiluted specimen, according to laboratory SOP |
| Storage | Carrying case for storage |
| Microscope type | Upright biological microscope with transmitted brightfield illumination |
| Recommended microscope | OPTIC900TL trinocular biological microscope |
| Observation magnification | Routine chamber scanning commonly uses 100x to 400x total magnification, depending on laboratory method |
| Microscope set range | OPTIC900TL standard magnification range 40x–1000x with standard objective set |
| Illumination | Even transmitted brightfield light; Koehler-style adjustment improves contrast and field uniformity |
| Stage recommendation | Mechanical XY stage helps repeatable movement across grid fields |
| Camera recommendation | Recommended for documentation, training, QC review and laboratory records |
| Cleaning note | Clean and dry optical surfaces after use; avoid scratching quartz glass and grid area |
This specification describes equipment configuration and workflow support. It does not define clinical reference limits, diagnostic interpretation, final SOP, validation procedure, sample acceptance criteria or reporting rules. Final chamber, microscope, camera, adapter, software, cleaning procedure and documentation requirements are confirmed in the official quotation.
STANDARD PACKAGING / SET CONFIGURATION
MAKLER100 chamber and optional complete microscope set
MAKLER100 chamber package
- MAKLER100 quartz glass counting chamber.
- Fixed 10 µm chamber depth with ruby spacers.
- 1 mm² grid with 100 squares.
- Carrying case for storage.
- Technical documentation according to supplied configuration.
Recommended complete set
- MAKLER100 sperm counting chamber.
- OPTIC900TL trinocular LED biological microscope.
- Compatible microscope camera.
- C-mount adapter / camera adapter according to selected camera.
- Documentation accessories and software options according to workflow.
Typical microscope workflow support
Preparation
Mix the specimen gently and bring chamber, cover and specimen handling to the laboratory’s required working condition according to SOP.
Loading
Apply the specimen so that the chamber fills correctly by capillary action, avoiding bubbles, overflow and inconsistent loading thickness.
Microscope setup
Use transmitted brightfield illumination, focus on the grid plane and adjust condenser and illumination for clear field contrast.
Documentation
A microscope camera can document selected fields for operator training, QC review and laboratory record support according to data policy.
RECOMMENDED MICROSCOPE SET
OPTIC900TL trinocular biological microscope with camera documentation workflow
The OPTIC900TL microscope platform supports the MAKLER100 workflow with transmitted brightfield observation, infinity plan achromatic optics, mechanical stage control and a camera-ready trinocular head. A compatible camera and software can help record microscope fields, support training and store QC reference images.
Optical platform
Infinity plan achromatic optics, standard objectives 4x, 10x, 40x(S) and 100x(S) oil, with 40x–1000x standard magnification range.
Illumination
5W LED Koehler transmitted illumination supports even brightfield observation and digital imaging.
Camera documentation
Trinocular photo port supports camera integration for field capture, training images and routine documentation.
OPTIONAL ACCESSORIES AND CONFIGURATION
Configure chamber, microscope, camera and documentation workflow
The final MAKLER100 set should be configured according to the laboratory’s procedure, reporting workflow and documentation requirements. Confirm whether the order should include only the chamber, or a complete microscope set with camera, adapter and software.
Camera and software
- Microscope camera for live image and field capture.
- C-mount or camera adapter according to selected camera.
- Image software according to reporting and storage workflow.
- Data policy and sample ID handling defined by the laboratory.
Microscope options
- OPTIC900TL trinocular LED microscope.
- Mechanical stage for repeatable XY field movement.
- Optional phase contrast and darkfield accessories for broader microscopy work.
- Additional objectives or filters if required by the wider application.
Laboratory procedure
- Validated semen-analysis SOP.
- Loading and timing rules.
- Cleaning and disinfection procedure compatible with optical glass.
- Operator training and QC image review procedure.
Ordering note: confirm chamber-only or complete set, microscope model, camera resolution, adapter, software, image storage policy, cleaning procedure and laboratory SOP before quotation.
MICROSCOPY CATALOGUE
Compare biological microscopes, cameras and microscopy accessories
Use the microscope overview to compare OPTIC900TL with other biological microscopes, routine brightfield systems, camera-ready microscopes and microscopy accessories for laboratory documentation workflows.
COLO.SCIENCE KNOWLEDGE AND SUPPORT
Support for chamber selection, microscope configuration and documentation workflow
COLO.Science can help define whether the laboratory needs MAKLER100 only, or a complete set with microscope, camera, C-mount adapter, documentation software and accessories.
How to select and configure MAKLER100
When selecting MAKLER100, first define the laboratory workflow: chamber-only replacement, new chamber and microscope set, or complete documentation set with camera and software. For routine brightfield observation, an upright biological microscope with transmitted illumination, stable mechanical stage and suitable magnification range is recommended.
The second selection step is documentation. If the laboratory needs image records for training, QC review or reporting support, select a microscope camera, C-mount adapter and compatible software together with the microscope. All counting rules, sample handling, cleaning and reporting should follow the laboratory’s validated SOP.
MAKLER100 sperm counting chamber for rapid microscope-based workflow support
MAKLER100 is a quartz glass sperm counting chamber with a fixed 10 µm chamber depth and a 1 mm² grid divided into 100 squares. It supports a microscope-based workflow for concentration counting and motility observation from an undiluted specimen, when used according to laboratory SOP and trained operator practice.
For complete use, the chamber can be paired with the OPTIC900TL trinocular LED biological microscope and a compatible microscope camera. This creates a practical set for brightfield observation, grid scanning, field capture, training records and QC documentation support. Clinical interpretation, result reporting and acceptance criteria remain the responsibility of the validated laboratory method.
Configuration notice
MAKLER100 sperm counting chamber and the recommended microscope set may be supplied in different configurations depending on chamber availability, microscope model, camera, C-mount adapter, software, accessories, documentation requirements and laboratory workflow. This page is provided for orientation and product selection. It does not replace the binding specification, delivered configuration, accessory set, validation protocol, clinical method, cleaning procedure or acceptance criteria confirmed in the official COLO.Science quotation, proforma invoice, order confirmation or manufacturer-approved technical offer. Specifications may change without notice.
