COLO.Science | Technical Specification
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MAKLER100 Sperm Counting Chamber

Quartz Glass Counting Chamber | 10 µm Depth | 1 mm² Grid | Sperm Concentration and Motility Workflow

MAKLER100 sperm counting chamber is a simple optical counting device for rapid sperm concentration and motility assessment from an undiluted specimen. The quartz glass optical path, fixed 10 µm chamber depth and 1 mm² counting grid help laboratories perform routine semen analysis workflow under a biological microscope.

Quartz glass10 µm depth1 mm² grid100 squares0.1 x 0.1 mmUndiluted specimenMotility checkStorage case
Document type: Technical Specification Sheet
Product family: Microscopy counting chambers / optical glass
Model: MAKLER100
Recommended set: MAKLER100 + OPTIC900TL trinocular LED microscope + microscope camera
Workflow: sperm concentration, motility observation and documentation support according to laboratory SOP
Format: A4 landscape | COLO.Science TechSpec v2.5
MAKLER100 sperm counting chamber
MAKLER100 chamber10 µm | 1 mm² grid | quartz glass
MAKLER100 sperm counting chamber | quartz glass optical path | fixed chamber depth
Counting area1 mm²
100 squares
Chamber depth10 µm
ruby spacers
Contactsales@colo.si
+386 70 713 437
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COLO.Science logoMAKLER100 | Sperm Counting Chamber
Technical data sheet | Informational document
Use with: biological microscope
MAKLER100 Request quote OPTIC900TL set PDF catalog Catalogue
MAKLER100 | rapid chamber-based semen analysis supportFixed-depth optical counting chamber for concentration and motility observation
10 µm chamberUndiluted sampleDirect countingMicroscope workflowDesigned for routine laboratory use; final interpretation follows validated laboratory procedure
Product Overview and Workflow
Counting chamber principle
Fixed optical depth: three ruby spacers provide a defined 10 µm chamber height for repeatable sample thickness.
Known grid area: the 1 mm² grid is subdivided into 100 squares of 0.1 x 0.1 mm for direct microscopic counting.
Undiluted specimen: the chamber is intended for rapid assessment from an undiluted semen specimen when used according to local SOP.
Quartz optical path: quartz glass supports clear transmitted-light observation and routine cleaning.
Spermogram elements supported
Concentration: direct count of cells in defined grid areas can support sperm concentration reporting.
Motility: live observation under brightfield microscopy supports progressive, non-progressive and immotile categorization according to laboratory method.
Vitality / morphology notes: chamber observation may support screening, while formal morphology or vitality assessment should follow the laboratory's validated staining and microscopy protocol.
Documentation: camera capture helps record fields, training examples and QC review images.
Typical operating sequence
Prepare: mix the specimen gently and bring chamber, cover and sample to the laboratory's required working temperature.
Load: apply a small specimen volume so the chamber fills by capillary action without bubbles or overflow.
Set microscope: use brightfield transmitted light, focus on the grid plane and adjust condenser/illumination for high contrast.
Count and classify: count selected grid areas and classify motility categories using a consistent field pattern and timing.
Quality-control considerations
Cleaning: clean optical glass immediately after use according to laboratory-compatible disinfectant and rinse procedure.
Repeatability: keep loading method, timing after loading, observation magnification and counting pattern consistent.
Training: use camera images and stored example fields to align operators on counting rules.
Limitations: this sheet describes equipment and workflow support; it does not define clinical reference limits or diagnostic interpretation.
MaterialQuartz glass
Depth10 µm
Grid1 mm² / 100 squares
Set optionOPTIC900TL + camera
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MAKLER100 Request quote OPTIC900TL set PDF catalog Catalogue
MAKLER100 Technical Specifications
Supplied COLO source values and chamber configuration
ModelProduct typeOptical materialChamber depthGrid areaGrid subdivisionSample typeStorage
MAKLER100Sperm counting chamberQuartz glass10 µm1 mm²100 squares, each 0.1 x 0.1 mmUndiluted specimenCarrying case
Chamber and optical system
ParameterSpecification
ModelMAKLER100
ApplicationRapid sperm count, motility observation and semen-analysis workflow support
Optical pathQuartz glass
Chamber spacing10 µm, provided by built-in three ruby spacers
Counting grid1 mm² grid
Grid subdivision100 squares, each 0.1 x 0.1 mm
SpecimenUndiluted specimen, according to laboratory SOP
StorageCarrying case for storage
Microscope and observation recommendations
ParameterPractical recommendation
Microscope typeUpright biological microscope with transmitted brightfield illumination
Observation magnificationRoutine chamber scanning commonly uses 100x to 400x total magnification depending on laboratory method
IlluminationEven transmitted light; Koehler-style adjustment improves contrast and field uniformity
StageMechanical XY stage helps repeatable movement across grid fields
CameraRecommended for documentation, training, QC review and customer/laboratory records
Temperature controlKeep specimen and chamber handling consistent with the laboratory's semen-analysis SOP
CleaningClean and dry optical surfaces after use; avoid scratching the quartz glass and grid area
Source note: MAKLER100 chamber values were transcribed from the supplied COLO product page and PDF. Workflow notes are general laboratory equipment guidance and must be aligned with local SOP, current semen-analysis standards and trained operator practice.
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MAKLER100 Request quote OPTIC900TL set PDF catalog Catalogue
Recommended Set: Chamber, OPTIC900TL Microscope and Camera
OPTIC900TL trinocular biological microscope with LED illumination
OPTIC900TL microscopeTrinocular | LED | camera port
OPTIC900TL trinocular biological microscope | LED transmitted illumination | camera documentation port
OPTIC900TL microscopy platform
Trinocular Siedentopf head: 30° inclined, 360° rotatable head with dedicated photo port for camera documentation.
Infinity plan achromatic optics: standard objectives 4x/0.10, 10x/0.25, 40x(S)/0.65 and 100x(S)/1.25 oil.
Magnification range: 40x to 1000x with WF10x/20 mm eyepieces and standard objective set.
5W LED Koehler illumination: adjustable transmitted illumination for brightfield observation and digital imaging.
Why pair it with MAKLER100?
Stable brightfield image: even LED illumination and plan optics support clear grid and cell observation.
Mechanical stage control: repeatable XY movement helps count defined chamber areas consistently.
Camera-ready workflow: trinocular port supports microscope cameras for screenshots, training, QC review and reports.
Expandable platform: optional phase contrast and darkfield accessories are available for broader microscopy work.
Suggested purchase set: MAKLER100 sperm counting chamber + OPTIC900TL trinocular LED biological microscope + compatible microscope camera/C-mount adapter. Confirm camera resolution, software needs and documentation workflow before quotation.
Chamber Kit and Documentation Images
MAKLER100 chamber in carrying case
Carrying caseMAKLER100 storage kit
MAKLER100 supplied with carrying case for storage
Camera and software selection
Routine documentation: select a microscope camera and adapter compatible with the trinocular photo port and reporting software.
Training: live camera image can support operator training and comparison of counting decisions.
Records: captured fields can be stored with sample ID according to the laboratory's data-protection and QC policy.
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COLO.Science | Product Support
MAKLER100 Request quote OPTIC900TL set PDF catalog Catalogue
Manufacturer and support
COLO Lab Experts
Polje ob Sotli 4, SI-3255, Slovenia
Selection guidance: send the intended semen-analysis workflow, preferred microscope configuration, camera/software requirement, documentation requirement and whether additional microscopy accessories are needed.
Quick contact
Web: colo.si
Product: MAKLER100 sperm counting chamber
COLO.Science | Semen-analysis microscopy set

Need a complete sperm counting chamber and microscope set?

COLO.Science can prepare a set with MAKLER100 chamber, OPTIC900TL trinocular LED microscope, compatible microscope camera and documentation accessories. Confirm the intended workflow, camera output, software needs and laboratory SOP before ordering.

Microscopy and analysis navigation

MAKLER100 Chamber OPTIC900TL Microscope

Set configuration

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Quote & technical support

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Official configuration notice: This document is provided for orientation and product selection. It does not replace the binding specification, delivered configuration, accessory set, validation protocol, clinical method or acceptance criteria confirmed in the official COLO.Science quotation, proforma invoice, order confirmation or manufacturer-approved technical offer. Confirm chamber, microscope, camera, adapter, software, cleaning procedure and documentation requirements for the specific order. Specifications may change without notice.
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