COLO.Science · Incubation & Controlled Environments · Molecular biology
ALHYB-OV hybridization oven — rotating bottle holder, RT+5 °C to 100 °C, 5–30 rpm, 99 programs
The ALHYB-OV hybridization oven is a benchtop oven for hybridization of membranes in rotating glass bottles. A stepper motor turns the holder at 5 to 30 rpm, so a few millilitres of probe solution wet the whole membrane, while PID control holds the chamber from RT+5 °C to 100 °C to ≤ ±0.3 °C at 37 °C and ≤ ±0.1 °C inside the bottles. The standard holder takes ten 35 × 300 mm bottles or twenty 35 × 150 mm bottles. Product code PRO7194.
Hybridization and washing in the same bottle
In blot hybridization a labelled probe has to find its matching sequence on a membrane, at a temperature that is held for hours. In a rotating bottle the membrane lies against the glass wall and the solution runs over it with every turn, so little probe is needed and the bottle stays closed. The ALHYB-OV hybridization oven does the hybridization and the washing steps in the same bottle, at the temperature you set.
Key characteristics
- RT+5 °C to 100 °C, display resolution 0.1 °C.
- ≤ ±0.3 °C at 37 °C in the chamber, ≤ ±0.1 °C inside the bottles.
- 5 to 30 rpm, quiet maintenance-free stepper motor.
- Bottle holder for 10 bottles 35 × 300 mm or 20 bottles 35 × 150 mm.
- Colour LCD — temperature, speed and time on one screen.
- 99 programs, timer from 1 min to 99 h 59 min or continuous.
- Glass door and stainless steel chamber.
- Auto preheating, auto resume after a power failure, over-heating protection.
Suitable for
- Southern, Northern and dot blot hybridization of membranes in rotating bottles.
- Screening of cloned genes and detection of specific sequences.
- Washing of membranes in the same bottle at a set temperature.
- Incubation of 1.5 ml to 50 ml tubes with rotation, on the optional tube holder.
- Staining and destaining of gels and membranes on the optional rocking platform.
Why a hybridization oven with rotating bottles
The ALHYB-OV hybridization oven replaces sealed bags in a water bath. The bottles are closed, so probe and wash solutions stay inside; rotation spreads a small volume over the whole membrane; and the air chamber keeps the bottles at the same temperature to ≤ ±0.1 °C at 37 °C.
The same chamber takes three inserts — the bottle holder, a tube holder or a rocking platform — so one instrument serves hybridization, tube incubation and gel staining.
Model, product code and holders
The ALHYB-OV hybridization oven is supplied with the hybridization bottle holder as standard. The tube holder and the rocking platform are optional and have their own product codes. One holder or the platform is in the chamber at a time. Quote and order with the COLO product code.
RT+5 °C to 100 °C · 5–30 rpm · 99 programs · AC 100–240 V · 25 kg
Holders
| PRO7195 | Hybridization bottle holder · 10 × 35 × 300 mm or 20 × 35 × 150 mm · standard, included |
| PRO7196 | Tube holder, 4 plates · per plate 22 × 1.5/2.0 ml, 8 × 10/15 ml or 6 × 50 ml · optional |
| PRO7197 | Rocking platform · 270 × 215 mm · optional |
The plate type of the tube holder is chosen with the order.
Technical specifications
Temperature, rotation and time
| Temperature setting range | 0 °C to 100 °C |
| Temperature control range | RT+5 °C to 100 °C |
| Temperature accuracy | ≤ ±0.3 °C at 37 °C |
| Uniformity inside the oven | ≤ ±0.3 °C at 37 °C |
| Uniformity inside the bottles | ≤ ±0.1 °C at 37 °C |
| Display resolution | 0.1 °C |
| Speed range | 5 to 30 rpm |
| Time range | 1 min to 99 h 59 min, or continuous |
| Programs | 99 |
General data
| Maximum load | 5 kg |
| Auto preheating | Yes |
| Constant temperature at the end of run | Yes |
| Auto resume | Yes |
| Power | 1000 W |
| Voltage | AC 100–240 V, 50/60 Hz |
| Fuse | 250 V 3 A, Ø 5 × 20 |
| Interior dimensions | 267 × 326 × 273 mm |
| Overall dimensions | 428 × 400 × 470 mm |
| Weight | 25 kg |
How a run works
Program
Set temperature, speed and time, or recall one of the 99 programs. The ALHYB-OV hybridization oven preheats the chamber.
Load and start
Roll the membrane into the bottle, add the solution, close the bottle and clip it into the holder. Balance the load, close the door and start.
End of run
An alarm sounds and the oven can go on holding the temperature. Change the solution for the washing steps or take the bottles out.
Build, controls and safe operation
The ALHYB-OV hybridization oven measures 428 × 400 × 470 mm and weighs 25 kg. The chamber is stainless steel, 267 × 326 × 273 mm, and the glass door shows the samples during the run. The supply is AC 100–240 V, 50/60 Hz, 1000 W.
The colour LCD shows temperature, speed and remaining time together. Temperature calibration and over-heating protection are built in, and a run resumes automatically when the supply returns after a power failure. Load the holder evenly and keep to the maximum load of 5 kg.
Standard delivery
- ALHYB-OV hybridization oven.
- Hybridization bottle holder.
- Mains cable.
- Operating manual.
- Declaration of conformity and warranty documentation.
Hybridization bottles, membranes, probes and reagents are not part of the instrument unless stated in the quotation. The delivered configuration for a specific order is the one confirmed in the COLO.Science quotation.
Knowledge base
Incubation and molecular biology on COLO.Science — our knowledge base.
Applications and operational value
The ALHYB-OV hybridization oven is used in molecular biology, genetics and diagnostic laboratories for Southern, Northern and dot blot hybridization, probe screening and the washing steps that follow.
With the tube holder it incubates 1.5 ml to 50 ml tubes with rotation, and with the rocking platform it stains and destains gels and membranes in trays.
Selection guide
- Method. Membrane hybridization in bottles, tube incubation or rocking — it decides the holder.
- Bottles. Length 300 mm or 150 mm, diameter 35 mm, and how many per run.
- Temperature. Hybridization and wash temperatures must lie within RT+5 °C to 100 °C.
- Tubes. For the tube holder choose the plate: 1.5/2.0 ml, 10/15 ml or 50 ml.
- Slides instead of membranes? The ALHYB runs FISH on 12 microscope slides.
- Bench space. The ALHYB-OV hybridization oven needs 428 × 400 mm and weighs 25 kg.
Configuration notice
This page is provided for orientation and product selection. It does not replace the binding specification, the delivered configuration, the validation protocol or the acceptance criteria confirmed in the official COLO.Science quotation, proforma invoice or order confirmation. Confirm the holder, the bottle or tube format and the mains plug of the ALHYB-OV hybridization oven for the specific order. B2B prices are quoted net, excluding VAT.
Sales and support for international customers
COLO Lab ExpertsPolje ob Sotli 4, SI-3255 Buče, Slovenia
Phone: +386 70 713 437 · Email: sales@colo.si · Web: colo.si
We supply laboratories across Europe directly and through partners. B2B prices are quoted net, excluding VAT.
