COLO.Science — LEGEND-250S UV/VIS DOUBLE BEAM SPECTROPHOTOMETER
LEGEND-250S — UV/Vis Double Beam scanning spectrophotometer, 190–1100 nm, 0.5/1/2/4/5 nm bandwidth
Research-grade UV/Vis double-beam spectrophotometer for routine and advanced laboratory analysis, with 10.1″ capacitive touch screen, fast scanning workflows, internal memory, and USB/SD export for reporting and QA/QC. Variable spectral bandwidth supports flexible method development and verification.
190–1100 nm
0.5/1/2/4/5 nm spectral bandwidth
10.1″ touch screen
Max 4,000 nm/min scan
USB + SD + Excel export
Silicon photodiode
D2 + tungsten-halogen
Wi-Fi UV lamp control (option)
COLO.SCIENCE – UV/VIS SPECTROPHOTOMETER / LEGEND SERIES
Why LEGEND-250S is a practical lab choice
LEGEND-250S is a double-beam UV/Vis scanning spectrophotometer designed for precision measurement in research and production workflows. It combines a stable optical design, fast measurement automation, and a 10.1″ capacitive touch screen for clear curves, parameters and reporting-ready results.
Its variable spectral bandwidth (0.5/1/2/4/5 nm) supports flexible method setup, sharper peak resolution when needed, and robust routine operation across a wide range of applications.
Key features (short)
- 190–1100 nm wavelength range for UV and visible methods.
- 0.5/1/2/4/5 nm variable spectral bandwidth for method flexibility.
- Multi-function modes: %T, Abs, C, E (photometric, quantitative, scan, kinetics, DNA/protein).
- Touchscreen UI with help menu + professional PC software for full data processing.
- USB host/drive + SD storage; export to Excel for reporting.
Where it fits best
- Routine UV/Vis methods and verification tests.
- Quantitative assays with calibration curves and direct concentration readout.
- Spectral scanning and peak/valley analysis for identification and QA.
- Environmental / water labs, food labs and industrial QC reporting.
PRACTICAL USE & CHECKLISTS
Daily workflow checklist (fast)
Warm up as per SOP, select method (photometric / quantitative / scan / kinetics), run auto blank and auto-zero, then measure samples. Use peak/valley detection and spectrum operations when running scans.
Save results to internal memory or USB/SD and export to Excel for QC reporting. For quant work, build a calibration curve and use direct concentration reading.
COLO.Science & equipment
COLO.Science helps labs select the right UV/Vis configuration (cuvette holders, multi-position holders, Peltier, sipper system, printers, software) and implement stable, repeatable measurement workflows.
If you need 8-position holders, variable optical path or compliance-oriented software, we propose a matched configuration for your application.
TECHNICAL SPECIFICATIONS — LEGEND-250S (CONFIGURATION DEPENDENT)
| Optical system | Double beam, optical grating 1200 lines/mm |
| Wavelength range | 190–1100 nm |
| Spectral bandwidth | 0.5 / 1 / 2 / 4 / 5 nm |
| Wavelength accuracy | ±0.3 nm (entire range) / ±0.1 nm (656.1 nm) |
| Wavelength repeatability | ≤0.1 nm |
| Photometric accuracy | ±0.0025 A at 1.0 Abs |
| Photometric repeatability | ±0.0002 at 0.5 A / ±0.0006 at 1.0 A / ±0.001 at 2.0 A |
| Photometric range | 0–400%T, -4 to +4A, 0–9999C (0–9999F) |
| Stray light | ≤0.02%T (at 220 nm) |
| Scan speed | Max 4,000 nm/min |
| Stability | ≤±0.0003 A/h at 500 nm |
| Baseline flatness | ±0.0001 A |
| Baseline stability | < 0.0003 Abs/h |
| Noise | 0.00005 Abs at 700 nm |
| Wavelength setting | Auto |
| Measuring mode | %T, Abs, C, E (photometric, quantitative, spectral scanning, kinetics, DNA/protein…) |
| Spectrum analysis / scanning | Subtraction/addition, peak/valley detection, selection, peak area, peak overlap |
| Light source | Deuterium (D2) / Tungsten-Halogen lamp |
| Scanning speed presets | Fast / Medium / Slow |
| Display | 10.1″ TFT color capacitive touch screen |
| Detector | Silicon photodiode |
| I/O | USB host / USB drive / RS232 / SD card |
| Power | AC220V, 50–60 Hz, 100W |
| Instrument size | 760 × 560 × 280 mm |
| Packing size | 880 × 690 × 520 mm |
| Gross / net weight | 28 / 47 kg |
| PDF reference | Open official TechSpec |
Included items (typ.)
- Spectrophotometer + Euro plug power cable
- 4 pcs glass cuvette + 2 pcs quartz cuvette
- Instruction manual
Options (typ.): built-in printer, professional LEGEND software, 5 mm to 100 mm variable optical path, up to 8-place cuvette holder, Wi-Fi UV lamp control (option).
Manufacturer reserves the right to change technical specifications.
LEGEND SERIES — QUICK COMPARISON (EDIT PARAMS HERE FOR OTHER PAGES)
| Type | LEGEND-100 | LEGEND-150 | LEGEND-150S | LEGEND-200 | LEGEND-250 | LEGEND-250S |
|---|---|---|---|---|---|---|
| Optical system | Single beam Optical grating 1200 lines/mm |
Single beam Optical grating 1200 lines/mm |
Single beam Optical grating 1200 lines/mm |
Double beam Optical grating 1200 lines/mm |
Double beam Optical grating 1200 lines/mm |
Double beam Optical grating 1200 lines/mm |
| Wavelength range (nm) | 190–1100 | 190–1100 | 190–1100 | 190–1100 | 190–1100 | 190–1100 |
| Spectral bandwidth (nm) | 2 | 1 | 0.5 / 1 / 2 / 4 / 5 | 2 | 1 | 0.5 / 1 / 2 / 4 / 5 |
| Wavelength accuracy | ±0.3 nm (for entire range) / ±0.1 nm (656.1 nm) | |||||
| Wavelength repeatability | ≤0.1 nm | |||||
| Photometric accuracy | ±0.0025 A at 1.0 Abs | |||||
| Photometric repeatability | ±0.0002 at 0.5 A / ±0.0006 at 1.0 A / ±0.001 at 2.0 A | |||||
| Photometric range | 0–400%T, -4 to +4A, 0–9999C (0–9999F) | |||||
| Stray light | ≤0.02%T (at 220 nm) | |||||
| Scan speed | Max 4,000 nm/min | |||||
| Stability | ≤±0.0005 A/h at 500 nm | ≤±0.0005 A/h at 500 nm | ≤±0.0003 A/h at 500 nm | ≤±0.0003 A/h at 500 nm | ||
| Baseline flatness | ±0.0002 A | — | ±0.0001 A | ±0.0002 A | — | ±0.0001 A |
| Noise | 0.00005 Abs at 700 nm | |||||
| Baseline stability | < 0.0003 Abs/h | |||||
| Wavelength setting | Auto | |||||
| Measuring mode | %T, Abs, C, E; Photometric, Quantitative, Spectral Scanning, Kinetics, DNA/protein… | |||||
| Full spectrum analyse / scanning mode | Subtraction/addition, Peak/Valley detection, selection, peak area, peak overlap | |||||
| Light source | Deuterium (D2) / Tungsten-Halogen lamp | |||||
| Wavelength scanning speed | Fast / Medium / Slow | |||||
| Display | 10.1″ TFT color capacitive touch screen | |||||
| Detector | Silicon photodiode | |||||
| Output | USB Host / USB Drive / RS232 / SD Card | |||||
| Power | AC220V / 50Hz, 100W | |||||
| Instrument size | 590 × 475 × 250 mm | 760 × 560 × 280 mm | — | 590 × 475 × 250 mm | 760 × 560 × 280 mm | — |
| Packing size | 810 × 660 × 390 mm | 880 × 690 × 520 mm | — | 810 × 660 × 390 mm | 880 × 690 × 520 mm | — |
| Gross / net weight (kg) | 20 / 33 | 28 / 47 | — | 20 / 33 | 28 / 47 | — |
| Standard configuration | Spectrophotometer, Euro plug power cable, 4 pcs glass cuvette, 2 pcs quartz cuvette, instruction manual | |||||
| Option | Built-in printer, professional LEGEND software, 5 mm to 100 mm variable optical path, up to 8-place cuvette holder | |||||
Tip: keep this table identical across LEGEND pages; only adjust the highlighted model column (bandwidth, stability, size/weight, etc.).
Configuration notice — spectrophotometers are delivered per application
Spectrophotometers are typically configured according to the user’s workflow and required accessories (cuvette holders, thermostating, sipper system, printer, compliance software). The official PDF technical specification defines the exact configuration, included components and available options for each model/variant. Website content is provided for general informational purposes and may not represent the final delivered configuration.
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