Pseudomonas aeruginosa · Real-time PCR
Pseudomonas aeruginosa qPCR Detection Kit – real-time PCR with fluorescent probe
The Pseudomonas aeruginosa qPCR Detection Kit detects Pseudomonas aeruginosa DNA by real-time PCR with a fluorescent probe after enrichment of the sample. The kit contains ready-to-use reaction mix, lysis solution and controls.
- Real-time PCR with fluorescent probe
- Detection limit about 100 CFU per test
- Result within hours after enrichment
How Pseudomonas aeruginosa qPCR Detection Kit works
After enrichment, cells are lysed at 99 °C for 10 minutes and the DNA is amplified with specific primers and probe in 40 cycles. The Ct value shows whether the target is present.
Typical results
| Result | Read-out |
| Positive | Ct ≤ 35 |
| Retest | Ct 35–37 |
| Negative | Ct ≥ 37 or no Ct |
Technical data
| Sample preparation | Membrane filter enriched in Pseudomonas aeruginosa enrichment broth, 36 ± 1 °C, 18–24 h; lysis 99 °C, 10 min |
| PCR program | 40 cycles; fluorescence read at 60 °C in each cycle |
| Storage | −20 °C, protected from light; avoid repeated freezing and thawing |
| Shelf life | 12 months |
Applications and practical notes
Rapid molecular tests detect Pseudomonas aeruginosa in enrichment cultures of water and product samples within hours, while the culture method of ISO 16266 remains the reference. Typical applications include:
- Screening of water samples after membrane filtration
- Bottled water and beverage production
- Cosmetic and pharmaceutical products
- Laboratories with PCR or LAMP equipment
Working with Pseudomonas aeruginosa qPCR Detection Kit: Open the reaction tubes only after the run in a separate area, or not at all, and run a positive and a negative control every time.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Pseudomonas methods: ISO 16266 describes the detection and enumeration of Pseudomonas aeruginosa in water by membrane filtration on CN agar, where blue-green colonies that produce pyocyanin are counted as confirmed and other typical colonies are confirmed with further tests. ISO 13720 gives the count of presumptive Pseudomonas spp. in meat and meat products on CFC agar at 25 °C. Pharmacopoeial tests for P. aeruginosa use Cetrimide Agar. COLO.Science offers media, supplements, rapid tests and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Pseudomonas aeruginosa qPCR Detection Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Pseudomonas aeruginosa qPCR Detection Kit, please send us a request. With every delivery of Pseudomonas aeruginosa qPCR Detection Kit you receive the lot number and expiry date, and on request we send the available documentation for Pseudomonas aeruginosa qPCR Detection Kit.
Where Pseudomonas aeruginosa qPCR Detection Kit fits in Pseudomonas testing
- Water – P. aeruginosa – membrane filtration on CN agar (Pseudomonas Agar Base with CN supplement), 36 °C, 44 h
- Food – Pseudomonas spp. – CFC agar (Pseudomonas Agar Base with CFC supplement), 25 °C, 44 h
- Isolation and screening – Pseudomonas Chromogenic Medium and Cetrimide Agar
- Confirmation and QC – qPCR or LAMP and P. aeruginosa reference strains
See all Pseudomonas products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Pseudomonas aeruginosa qPCR Detection Kit | PRO7388 | 48 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
