Vibrio · Biochemical identification
Vibrio parahaemolyticus Identification Kit – biochemical confirmation of typical colonies
The Vibrio parahaemolyticus Biochemical Identification Kit confirms typical colonies from TCBS or chromogenic agar with two strips of miniaturised biochemical tests prepared in saline.
- Two strips of biochemical tests per sample
- Prepared in saline for halophilic vibrios
- 5 tests
How Vibrio parahaemolyticus Identification Kit works
A pure colony is suspended to 0.5 McFarland and dispensed into the wells. Fermentation of glucose and mannitol, lysine decarboxylation and other reactions are compared with the profile of V. parahaemolyticus, together with the oxidase test.
Typical results
| Organism | Typical profile |
| V. parahaemolyticus | Oxidase +, glucose +, mannitol +, lysine +, sucrose −, lactose −, H₂S −, VP −, ONPG − |
Technical data
| Inoculum | Pure colony suspended to 0.5 McFarland |
| Incubation | 36 ± 1 °C, 24 h |
| Storage | 2–8 °C |
Applications and practical notes
Rapid tests shorten the detection of Vibrio after enrichment, while positive results are confirmed by the culture method of ISO 21872-1. Typical applications include:
- Screening of seafood batches
- Aquaculture monitoring
- Laboratories that need results within a working day
- Confirmation of suspect colonies
Working with Vibrio parahaemolyticus Identification Kit: Use a pure culture from T1N1 Agar, overlay the wells that need paraffin and read the oxidase test separately within 30 seconds.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Vibrio method: ISO 21872-1 describes the detection of potentially enteropathogenic Vibrio parahaemolyticus, V. cholerae and V. vulnificus in seafood and other foods. Samples are enriched twice in Alkaline Saline Peptone Water, plated on TCBS Agar and a second selective medium, and typical colonies are purified on saline agar and confirmed biochemically or by PCR. The FDA BAM Chapter 9 follows a similar scheme. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Vibrio parahaemolyticus Identification Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Vibrio parahaemolyticus Identification Kit, please send us a request. With every delivery of Vibrio parahaemolyticus Identification Kit you receive the lot number and expiry date, and on request we send the available documentation for Vibrio parahaemolyticus Identification Kit.
Where Vibrio parahaemolyticus Identification Kit fits in Vibrio testing
- Enrichment – Alkaline Saline Peptone Water (ASPW), 37 °C and 41.5 °C
- Isolation – TCBS Agar plus a second medium: chromogenic Vibrio agar, mCPC or CC agar
- Purification – non-selective saline agar such as T1N1 Agar
- Confirmation – oxidase, biochemical identification, PCR or LAMP
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Vibrio parahaemolyticus Identification Kit | PRO7891 | 5 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
