Cronobacter · Conventional PCR
Cronobacter Duplex PCR Kit – two targets, gel read-out
The Cronobacter Duplex PCR Kit identifies Cronobacter sakazakii by conventional PCR with two specific targets in one reaction. The products are read on an agarose gel, so the kit suits laboratories with a standard thermocycler.
- Two specific targets in one reaction
- Freeze-dried reaction mix
- Read-out by gel electrophoresis
How Cronobacter Duplex PCR Kit works
After thermal lysis, the DNA is added to the freeze-dried reaction mix. Amplification takes about 1.5 hours, and two bands at the expected positions on the gel indicate C. sakazakii.
Typical results
| Result | Read-out |
| Positive | Two specific bands at the target positions |
| Negative | No specific bands |
Technical data
| Format | Freeze-dried reaction mix in strips (3 × 8 tubes) with controls |
| Run time | Lysis about 10 min, PCR about 1.5 h, gel about 1 h |
| Detection limit | About 300 CFU per test |
Applications and practical notes
Rapid tests shorten the time to result for Cronobacter after enrichment, while positive results are confirmed by the culture method of ISO 22964. Typical applications include:
- Screening of infant formula batches before release
- Environmental monitoring in powder plants
- Laboratories that need results within a working day
- Confirmation of suspect colonies
Working with Cronobacter Duplex PCR Kit: Load the positive and negative controls on every gel and compare band positions with the size marker.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Cronobacter method: ISO 22964 is the horizontal method for Cronobacter spp. in infant formula, infant cereals, milk powders and production environments. It combines pre-enrichment in Buffered Peptone Water, selective enrichment at 41.5 °C, plating on a chromogenic agar and biochemical confirmation; the FDA BAM Chapter 29 uses a similar culture and PCR approach. COLO.Science offers media, supplements, rapid tests and identification kits for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Cronobacter Duplex PCR Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Cronobacter Duplex PCR Kit, please send us a request. With every delivery of Cronobacter Duplex PCR Kit you receive the lot number and expiry date, and on request we send the available documentation for Cronobacter Duplex PCR Kit.
Where Cronobacter Duplex PCR Kit fits in Cronobacter testing
- Pre-enrichment – Buffered Peptone Water, 34–38 °C
- Selective enrichment – Cronobacter enrichment broth with vancomycin-type supplement at 41.5 °C
- Isolation – Chromogenic Cronobacter Agar: blue-green colonies
- Confirmation – biochemical identification; rapid screening by qPCR, LAMP or detection plate
See all Cronobacter products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Cronobacter Duplex PCR Kit | PRO7421 | 24 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
