Pharmacopoeial media · Sample diluent
Buffered Sodium Chloride-Peptone Solution – pharmacopoeial diluent pH 7.0
Buffered Sodium Chloride-Peptone Solution pH 7.0 is the diluent of the pharmacopoeias for preparing samples of non-sterile products. It is used for initial suspensions, dilution series and rinsing of membrane filters, and can be supplemented with polysorbate 80 for fatty samples.
- Pharmacopoeial diluent, pH 7.0
- Initial suspensions and dilution series
- Rinsing of membrane filters
How Buffered Sodium Chloride-Peptone Solution works
Phosphate buffer holds the pH at 7.0, sodium chloride keeps the osmotic balance and a small amount of peptone protects the cells, so counts remain stable during sample preparation.
Technical data
| Preparation | 14.63 g/L; boil to dissolve, dispense, autoclave 121 °C 15 min |
| Final pH | 7.0 ± 0.2 |
| Performance | Colony count stable within ± 50 % after 45 min holding |
| Storage | Dry, dark place; keep the container tightly closed |
| Shelf life | 3 years |
Applications and practical notes
Pharmacopoeial testing starts with sample preparation: the product is dissolved or suspended in a buffered diluent, and preservatives or antimicrobial residues are neutralised with lecithin and polysorbate so that surviving micro-organisms can grow (Ph. Eur. 2.6.12, USP <61>). Typical applications include:
- Preparation of initial suspensions and dilutions
- Neutralisation of preservatives and disinfectants
- Rinsing of membrane filters
- Pharmaceutical and cosmetic laboratories
Working with Buffered Sodium Chloride-Peptone Solution: Add polysorbate 80 or another neutraliser when the product contains preservatives, and confirm recovery in the method suitability test.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About pharmacopoeial microbiological testing: the European Pharmacopoeia and the USP describe harmonised methods for non-sterile products – microbial enumeration (Ph. Eur. 2.6.12, USP <61>) and tests for specified micro-organisms (Ph. Eur. 2.6.13, USP <62>) – and the sterility test for sterile products (Ph. Eur. 2.6.1, USP <71>). The chapters define the media, incubation conditions and the growth promotion test that each batch of medium must pass with reference strains. COLO.Science offers the media, diluents, neutralisers and QC strains for these tests from one source.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Buffered Sodium Chloride-Peptone Solution is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Buffered Sodium Chloride-Peptone Solution, please send us a request. With every delivery of Buffered Sodium Chloride-Peptone Solution you receive the lot number and expiry date, and on request we send the available documentation for Buffered Sodium Chloride-Peptone Solution.
Where Buffered Sodium Chloride-Peptone Solution fits in pharmacopoeial testing
- Sample preparation – Buffered Sodium Chloride-Peptone Solution, Tryptic Soy Broth and neutralising diluents
- Microbial enumeration – Tryptic Soy Agar (TAMC) and Sabouraud Dextrose Agar (TYMC)
- Specified micro-organisms – enrichment in Tryptic Soy Broth, then MacConkey, Mannitol Salt, Cetrimide, XLD, VRBG, RCM and Columbia media
- Sterility test – Fluid Thioglycollate Medium and Tryptic Soy Broth, 14 days
See all pharmacopoeial media in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Buffered Sodium Chloride-Peptone Solution pH 7.0 | PRO7481 | 500 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
