Supplement · Ampicillin for MacConkey-type agar
Ampicillin MacConkey Agar Supplement – selective antibiotic supplement
Ampicillin MacConkey Agar Supplement contains ampicillin for MacConkey-type agar. It is used when only ampicillin-resistant Gram-negative bacteria should grow on the plate.
- 2 mg ampicillin per vial
- One vial for 100 mL of base
- Selective for ampicillin-resistant bacteria
How Ampicillin MacConkey Agar Supplement works
Ampicillin inhibits susceptible bacteria, so only resistant strains form colonies. The MacConkey base still shows lactose fermentation by colony colour.
Technical data
| Preparation | 2 mg ampicillin/vial; dissolve in 1 mL sterile water, add to 100 mL base cooled to ~50 °C |
| Storage | 2-8 °C, protected from light after opening |
Applications and practical notes
Plate count and isolation media for coliforms and E. coli are used in food, feed, water and hygiene testing, for example according to ISO 4832 and ISO 16649-2. Typical applications include:
- Coliform and E. coli colony counts in food and feed
- Isolation of lactose-fermenting Gram-negative bacteria
- Process hygiene criteria in dairy, meat and vegetable production
- Teaching and routine quality-control laboratories
Working with Ampicillin MacConkey Agar Supplement: Dissolve each vial in 1 mL of sterile water and add it to 100 mL of base cooled to about 50 °C.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About coliform and E. coli methods: coliforms and E. coli are counted either by the multiple-tube (MPN) method, with a presumptive broth followed by confirmation broths, or by colony counts on selective agars. FDA BAM Chapter 4 describes both approaches for food and water, and ISO 16649-2 gives the colony-count method for β-glucuronidase-positive E. coli on TBX Agar. E. coli O157 and other STEC need dedicated media, serology and PCR. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Ampicillin MacConkey Agar Supplement is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Ampicillin MacConkey Agar Supplement, please send us a request. With every delivery of Ampicillin MacConkey Agar Supplement you receive the lot number and expiry date, and on request we send the available documentation for Ampicillin MacConkey Agar Supplement.
Where Ampicillin MacConkey Agar Supplement fits in coliform and E. coli testing
- Presumptive test – Lauryl Sulfate Tryptose Broth (MPN) or VRBL / chromogenic agar (colony count)
- Confirmation – BGLB Broth for coliforms; EC or EC-MUG Broth at 44.5 °C for E. coli
- Isolation – EMB, MacConkey, TBX or chromogenic E. coli agar
- Identification – IMViC kit; for O157 and STEC: SMAC or chromogenic O157 agar, latex and PCR
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Ampicillin MacConkey Agar Supplement | PRO7710 | 10 vials |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
