Listeria detection · rapid screening
Listeria monocytogenes qPCR Detection Kit – real-time PCR with fluorescent probe
The Listeria monocytogenes qPCR Detection Kit detects L. monocytogenes DNA by real-time PCR with a fluorescent probe after enrichment, on standard real-time PCR instruments.
- Real-time PCR with fluorescent probe
- Positive and negative controls included
- 48 tests per box · COLO code PRO7394
How the Listeria monocytogenes qPCR Detection Kit works
After enrichment, crude DNA is released by heating. The ready-to-use reagents contain primers and a probe specific for L. monocytogenes; the cycle threshold (Ct) shows whether the target DNA is present.
Reading the result
| Result | Ct value |
| Positive | Ct ≤ 35 |
| Negative | Ct ≥ 37 |
| Retest | Ct between 35 and 37 |
Positive results are confirmed by the culture method.
Technical data
| Enrichment | 30 ± 1 °C, 18–24 h |
| Sample preparation | Release DNA by lysis at 99 °C for 10 min |
| PCR programme | 95 °C 30 s; 40 cycles of 95 °C 5 s and 60 °C 30 s |
| Detection limit | 100 CFU per test |
| Storage | −20 °C, protected from light; avoid repeated freeze–thaw |
| Shelf life | 12 months |
Applications and practical notes
The kit is used in laboratories with real-time PCR instruments for rapid screening for L. monocytogenes; positives are confirmed according to ISO 11290-1. Typical applications include:
- ready-to-eat food producers
- dairy and cheese producers
- fish and meat processing
- environmental monitoring programmes
Working with Listeria monocytogenes qPCR Detection Kit: Keep the reagents frozen and protected from light, avoid repeated freezing and thawing, and run the kit controls with each series.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Listeria culture method: the reference method for Listeria monocytogenes in the food chain, ISO 11290-1, uses primary enrichment in Half-Fraser Broth, secondary enrichment in Fraser Broth, plating on a chromogenic agar of the Ottaviani-Agosti type and a second selective agar, and confirmation of typical colonies. The FDA BAM Chapter 10 uses Buffered Listeria Enrichment Broth instead. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Listeria monocytogenes qPCR Detection Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Listeria monocytogenes qPCR Detection Kit, please send us a request. With every delivery of Listeria monocytogenes qPCR Detection Kit you receive the lot number and expiry date, and on request we send the available documentation for Listeria monocytogenes qPCR Detection Kit.
Where the Listeria monocytogenes qPCR Detection Kit fits in the workflow
- Enrichment – Half-Fraser Broth or another Listeria enrichment
- Rapid screening – detection plate, LAMP or real-time PCR
- Confirmation – chromogenic Listeria agar, TSYEA, identification kit and latex
- Report – negative results reported quickly; positives confirmed
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Listeria monocytogenes qPCR Detection Kit | PRO7394 | 48 tests |
| Listeria monocytogenes LAMP Test Kit | PRO7411 | 24 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
