Vibrio · Selective isolation
CPC Agar Base – base for mCPC and CC agar
CPC Agar Base (CC Agar Base) is the base for cellobiose-polymyxin-colistin agars (mCPC and CC agar), the selective media for Vibrio vulnificus. It is completed with the mCPC or the CC Agar Supplement.
- Base for mCPC and CC agar
- Bromothymol blue and cresol red
- For Vibrio vulnificus
How CPC Agar Base works
The salt-containing nutrient base supports halophilic vibrios. Cellobiose and the polymyxins come from the supplement: V. vulnificus ferments cellobiose and forms yellow colonies, while most other vibrios and Gram-negative bacteria are suppressed.
Technical data
| Preparation | 50.08 g/L; heat with stirring to boiling, autoclave 121 °C 15 min; cool to about 50 °C and add the supplement |
| Final pH | 7.6 ± 0.2 |
| Storage | Cool, dry place away from bright light; keep the container tightly closed |
| Shelf life | 3 years |
Applications and practical notes
Pathogenic vibrios are tested in seafood, fish and coastal water, for example according to ISO 21872-1. Typical applications include:
- Fish, shellfish and seafood
- Coastal and brackish water
- Aquaculture
- Clinical and public-health laboratories
Working with CPC Agar Base: Add the mCPC or CC supplement only after the sterile base has cooled to about 50 °C.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Vibrio method: ISO 21872-1 describes the detection of potentially enteropathogenic Vibrio parahaemolyticus, V. cholerae and V. vulnificus in seafood and other foods. Samples are enriched twice in Alkaline Saline Peptone Water, plated on TCBS Agar and a second selective medium, and typical colonies are purified on saline agar and confirmed biochemically or by PCR. The FDA BAM Chapter 9 follows a similar scheme. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
CPC Agar Base is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include CPC Agar Base, please send us a request. With every delivery of CPC Agar Base you receive the lot number and expiry date, and on request we send the available documentation for CPC Agar Base.
Where CPC Agar Base fits in Vibrio testing
- Enrichment – Alkaline Saline Peptone Water (ASPW), 37 °C and 41.5 °C
- Isolation – TCBS Agar plus a second medium: chromogenic Vibrio agar, mCPC or CC agar
- Purification – non-selective saline agar such as T1N1 Agar
- Confirmation – oxidase, biochemical identification, PCR or LAMP
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| CPC Agar Base (CC Agar Base) | PRO8174 | 500 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
