Campylobacter · Cultivation
Brucella Broth – cultivation of Campylobacter
Brucella Broth is a nutrient broth for the cultivation of fastidious bacteria and is used in particular for the cultivation and maintenance of Campylobacter strains.
- For cultivation of Campylobacter
- Casein and tissue hydrolysates, yeast extract
- Liquid medium
How Brucella Broth works
Casein and animal tissue hydrolysates, yeast extract and glucose provide nutrients and growth factors, and sodium bisulphite helps to reduce oxygen tension, which supports microaerophilic organisms such as Campylobacter.
Technical data
| Preparation | 28.1 g/L; heat with stirring to boiling, autoclave 121 °C 15 min |
| Final pH | 7.0 ± 0.2 |
| Storage | Cool, dry place away from bright light; keep the container tightly closed |
| Shelf life | 3 years |
Applications and practical notes
Campylobacter is tested in poultry, meat, milk and water, for example according to ISO 10272-1. Typical applications include:
- Poultry meat and carcass rinses
- Raw milk and dairy products
- Drinking and surface water
- Environmental and faecal samples
Working with Brucella Broth: Incubate Campylobacter cultures in a microaerobic atmosphere and subculture regularly, because the organisms lose viability quickly in air.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Campylobacter method: ISO 10272-1 describes the detection of thermotolerant Campylobacter in food and feed. Samples with low numbers and stressed cells are enriched in Bolton broth, samples with high background flora in Preston broth. Enrichment cultures are plated on mCCD agar and a second selective agar of the laboratory’s choice and incubated microaerobically at 41.5 °C. Typical colonies are confirmed by morphology, motility, oxidase and further tests. COLO.Science offers supplements, media and latex kits for these steps.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Brucella Broth is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Brucella Broth, please send us a request. With every delivery of Brucella Broth you receive the lot number and expiry date, and on request we send the available documentation for Brucella Broth.
Where Brucella Broth fits in Campylobacter testing
- Enrichment – Bolton broth or Preston broth with selective supplement, microaerobic
- Selective isolation – mCCD agar plus a second medium such as Skirrow or Karmali agar, 41.5 °C
- Confirmation – morphology, oxidase and latex agglutination
- Media QC – Campylobacter reference strains
See all Campylobacter products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Brucella Broth | PRO8172 | 500 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
