Legionella pneumophila · Real-time PCR
Legionella pneumophila qPCR Detection Kit – real-time PCR with fluorescent probe
The Legionella pneumophila qPCR Detection Kit detects Legionella pneumophila DNA in water samples by real-time PCR with a fluorescent probe. It gives a result on the same day, while culture takes up to 10 days.
- Real-time PCR with fluorescent probe
- Detection limit about 100 CFU per test
- Same-day result
How Legionella pneumophila qPCR Detection Kit works
Cells concentrated from the water sample are lysed by heating and the DNA is amplified with specific primers and probe in 40 cycles. The Ct value shows whether L. pneumophila is present.
Typical results
| Result | Read-out |
| Positive | Ct ≤ 36 |
| Retest | Ct 36–39 |
| Negative | Ct ≥ 39 or no Ct |
Technical data
| Sample preparation | Concentrated sample in 30 µL lysis buffer, 100 °C, 5–10 min |
| PCR program | 40 cycles; fluorescence read at 60 °C in each cycle |
| Storage | −20 °C; avoid repeated freezing and thawing |
| Shelf life | 12 months |
Applications and practical notes
Rapid tests and latex kits shorten the confirmation of Legionella from water samples, while culture according to ISO 11731 remains the reference. Typical applications include:
- Fast screening of water samples
- Serogroup identification of isolates
- Outbreak investigations
- Laboratories with PCR or LAMP equipment
Working with Legionella pneumophila qPCR Detection Kit: Open the reaction tubes only after the run in a separate area, or not at all, and run a positive and a negative control every time.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Legionella method: ISO 11731 describes the enumeration of Legionella in water by direct plating or membrane filtration on BCYE and the selective GVPC agar, with incubation at 36 °C for up to 10 days. Presumptive colonies are confirmed by their need for L-cysteine: they grow on BCYE agar but not on BCYE agar without cysteine. L. pneumophila and its serogroups are then identified by latex agglutination. COLO.Science offers media, supplements, latex kits and rapid tests for these steps.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Legionella pneumophila qPCR Detection Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Legionella pneumophila qPCR Detection Kit, please send us a request. With every delivery of Legionella pneumophila qPCR Detection Kit you receive the lot number and expiry date, and on request we send the available documentation for Legionella pneumophila qPCR Detection Kit.
Where Legionella pneumophila qPCR Detection Kit fits in Legionella testing
- Sample preparation – membrane filtration, with heat or acid treatment for samples with high background flora
- Culture – GVPC agar and BCYE agar, 36 °C, up to 10 days
- Confirmation – subculture on BCYE and on BCYE without cysteine
- Identification – latex agglutination; qPCR or LAMP for fast screening
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Legionella pneumophila qPCR Detection Kit | PRO7402 | 48 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
