Shigella · QC reference strain
Shigella dysenteriae QC Strain – CMCC(B) 51105 reference strain
Shigella dysenteriae CMCC(B) 51105 is a qualitative reference strain for growth-promotion testing of Shigella media, for positive controls in the detection method and for checking identification kits.
- Lyophilised, single use
- For growth-promotion and controls
- Recovery fluid included
How Shigella dysenteriae QC Strain is used
The lyophilised culture is rehydrated with 1 mL of the supplied fluid, spread on a non-selective agar and subcultured. Purity and typical colony appearance are checked before use.
Technical data
| Preparation | Add 1.0 mL of the supplied recovery fluid, vortex and spread 0.1 mL on a non-selective plate or into broth |
| Incubation | 36 ± 1 °C, 24–48 h |
| Storage | As stated on the label; use the rehydrated suspension within 8 hours (2–8 °C) |
Applications and practical notes
Rapid tests, identification kits and reference strains shorten and secure the detection of Shigella after enrichment, while the culture method of ISO 21567 remains the reference. Typical applications include:
- Screening of enrichment cultures
- Confirmation of typical colonies
- Positive controls and media QC
- Laboratories with PCR or LAMP equipment
Working with Shigella dysenteriae QC Strain: Subculture the strain no more than five times from the original vial and record the passage number.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Shigella and Yersinia methods: ISO 21567 describes the detection of Shigella in food by enrichment in Shigella broth with novobiocin, plating on selective agars such as MacConkey, XLD and Hektoen enteric agar, and biochemical and serological confirmation. ISO 10273 describes the detection of presumptive pathogenic Yersinia enterocolitica by enrichment at 25 °C and plating on CIN agar, followed by tests that separate pathogenic from non-pathogenic strains. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Shigella dysenteriae QC Strain is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Shigella dysenteriae QC Strain, please send us a request. With every delivery of Shigella dysenteriae QC Strain you receive the lot number and expiry date, and on request we send the available documentation for Shigella dysenteriae QC Strain.
Where Shigella dysenteriae QC Strain fits in Shigella and Yersinia testing
- Enrichment – Shigella broth with selective supplement; for Yersinia a peptone-sorbitol-bile type broth
- Selective isolation – Chromogenic Shigella Agar, Desoxycholate Citrate Agar, XLD; for Yersinia CIN agar and Chromogenic Yersinia enterocolitica Agar
- Screening – qPCR or LAMP kits after enrichment
- Confirmation and QC – biochemical identification kits and reference strains
See all Shigella and Yersinia products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Shigella dysenteriae QC Strain CMCC(B) 51105 | PRO7855 | 1 vial |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
