Streptococci · Lancefield grouping
Streptococcus Grouping Latex Kit – Lancefield groups A, B, C, D, F and G
The Streptococcus Grouping Latex Kit identifies β-haemolytic streptococci by their Lancefield group antigen (A, B, C, D, F and G) with latex agglutination after a short extraction of colonies.
- Groups A, B, C, D, F and G
- Extraction of colonies from blood agar
- 50 tests
How Streptococcus Grouping Latex Kit works
The group-specific carbohydrate antigen is extracted from a few colonies. Each latex reagent is coated with antibodies against one group, and visible agglutination with one reagent shows the group of the strain.
Typical results
| Result | Read-out |
| Positive | Agglutination with one group reagent |
| Negative | No agglutination |
Applications and practical notes
Rapid tests and latex kits shorten the identification of enterococci and streptococci after culture or enrichment. Typical applications include:
- Confirmation of typical colonies
- Screening of water samples after enrichment
- Clinical and veterinary laboratories
- Laboratories with PCR or LAMP equipment
Working with Streptococcus Grouping Latex Kit: Use colonies from a fresh blood agar culture and test the extract with all latex reagents of the kit.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Enterococcus methods: intestinal enterococci are indicators of faecal contamination of water. ISO 7899-2 counts them by membrane filtration on Slanetz and Bartley Agar, where typical colonies are red, maroon or pink, and confirms them by transferring the membrane to Bile Esculin Azide Agar, where esculin hydrolysis turns the medium black. In food, enterococci are counted on bile esculin azide media. Streptococci are cultivated on blood agar, where the type of haemolysis is read, and grouped by Lancefield antigens. COLO.Science offers media, supplements, rapid tests and identification kits for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Streptococcus Grouping Latex Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Streptococcus Grouping Latex Kit, please send us a request. With every delivery of Streptococcus Grouping Latex Kit you receive the lot number and expiry date, and on request we send the available documentation for Streptococcus Grouping Latex Kit.
Where Streptococcus Grouping Latex Kit fits in Enterococcus and Streptococcus testing
- Enumeration in water – membrane filtration on Slanetz and Bartley Agar
- Confirmation – transfer of the membrane to Bile Esculin Azide Agar; Bile Esculin Agar for single colonies
- Streptococci – Blood Agar Base with blood for haemolysis, Mitis Salivarius Agar with potassium tellurite
- Identification – Lancefield grouping latex, qPCR or LAMP for Enterococcus faecalis
See all Enterococcus and Streptococcus products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Streptococcus Grouping Latex Kit (A, B, C, D, F, G) | PRO7899 | 50 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
