Enterococcus faecalis · Isothermal LAMP
Enterococcus faecalis LAMP Test Kit – visual result in under one hour
The Enterococcus faecalis LAMP Test Kit detects Enterococcus faecalis DNA by loop-mediated isothermal amplification. The reaction runs at one temperature in a simple heating block, and the result is read by colour.
- Isothermal amplification at 64 °C
- Visual read-out: green = positive
- Detection limit about 9 CFU per test
How Enterococcus faecalis LAMP Test Kit works
After enrichment and lysis at 99 °C, the sample is added to the lyophilised reaction tube. Specific primers amplify the target DNA in about 50 minutes. Positive reactions show green fluorescence, negative ones remain light orange.
Typical results
| Result | Read-out |
| Positive | Fluorescent green |
| Negative | Light orange |
Technical data
| Preparation | Lyophilised reagents; 20 µL reconstitution solution per reaction tube; sample lysis 99 °C, 10 min |
| Incubation | 64 °C (range 60–65 °C), 50 min |
| Storage | 2–8 °C |
| Shelf life | 12 months |
Applications and practical notes
Rapid tests and latex kits shorten the identification of enterococci and streptococci after culture or enrichment. Typical applications include:
- Confirmation of typical colonies
- Screening of water samples after enrichment
- Clinical and veterinary laboratories
- Laboratories with PCR or LAMP equipment
Working with Enterococcus faecalis LAMP Test Kit: Open the reaction tubes only after the run in a separate area, or not at all, and run a positive and a negative control every time.
Good practice with rapid tests: store the kit as stated on the label, let the components reach room temperature before use and use each item only once. Record the lot number and the result, and confirm positive results according to the reference method when this is required.
About the Enterococcus methods: intestinal enterococci are indicators of faecal contamination of water. ISO 7899-2 counts them by membrane filtration on Slanetz and Bartley Agar, where typical colonies are red, maroon or pink, and confirms them by transferring the membrane to Bile Esculin Azide Agar, where esculin hydrolysis turns the medium black. In food, enterococci are counted on bile esculin azide media. Streptococci are cultivated on blood agar, where the type of haemolysis is read, and grouped by Lancefield antigens. COLO.Science offers media, supplements, rapid tests and identification kits for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Enterococcus faecalis LAMP Test Kit is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Enterococcus faecalis LAMP Test Kit, please send us a request. With every delivery of Enterococcus faecalis LAMP Test Kit you receive the lot number and expiry date, and on request we send the available documentation for Enterococcus faecalis LAMP Test Kit.
Where Enterococcus faecalis LAMP Test Kit fits in Enterococcus and Streptococcus testing
- Enumeration in water – membrane filtration on Slanetz and Bartley Agar
- Confirmation – transfer of the membrane to Bile Esculin Azide Agar; Bile Esculin Agar for single colonies
- Streptococci – Blood Agar Base with blood for haemolysis, Mitis Salivarius Agar with potassium tellurite
- Identification – Lancefield grouping latex, qPCR or LAMP for Enterococcus faecalis
See all Enterococcus and Streptococcus products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Enterococcus faecalis LAMP Test Kit | PRO7406 | 24 tests |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
