Pseudomonas aeruginosa · Supplement
CN Supplement – cetrimide and nalidixic acid for CN agar
The CN Supplement turns Pseudomonas Agar Base into CN agar, the selective medium for Pseudomonas aeruginosa in water by membrane filtration according to ISO 16266.
- Cetrimide and nalidixic acid
- For CN agar according to ISO 16266
- 10 vials
How CN Supplement works
Cetrimide, a quaternary ammonium compound, and nalidixic acid inhibit most accompanying bacteria, while P. aeruginosa grows and forms its typical pigments on the base.
Applications and practical notes
Pseudomonas aeruginosa is an opportunistic pathogen of drinking, bottled and pool water, and Pseudomonas spp. are the main spoilage bacteria of chilled meat and fish. Typical applications according to ISO 16266 and ISO 13720 include:
- Drinking water, bottled and mineral water
- Swimming pool and spa water
- Meat, poultry and fish (spoilage flora)
- Pharmaceutical and cosmetic raw materials
Working with CN Supplement: Reconstitute the vial as stated on the label and add it to the sterile base after it has cooled to about 50 °C.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Pseudomonas methods: ISO 16266 describes the detection and enumeration of Pseudomonas aeruginosa in water by membrane filtration on CN agar, where blue-green colonies that produce pyocyanin are counted as confirmed and other typical colonies are confirmed with further tests. ISO 13720 gives the count of presumptive Pseudomonas spp. in meat and meat products on CFC agar at 25 °C. Pharmacopoeial tests for P. aeruginosa use Cetrimide Agar. COLO.Science offers media, supplements, rapid tests and QC strains for these methods.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
CN Supplement is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include CN Supplement, please send us a request. With every delivery of CN Supplement you receive the lot number and expiry date, and on request we send the available documentation for CN Supplement.
Where CN Supplement fits in Pseudomonas testing
- Water – P. aeruginosa – membrane filtration on CN agar (Pseudomonas Agar Base with CN supplement), 36 °C, 44 h
- Food – Pseudomonas spp. – CFC agar (Pseudomonas Agar Base with CFC supplement), 25 °C, 44 h
- Isolation and screening – Pseudomonas Chromogenic Medium and Cetrimide Agar
- Confirmation and QC – qPCR or LAMP and P. aeruginosa reference strains
See all Pseudomonas products in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| CN Supplement for Pseudomonas Agar | PRO7735 | 10 vials |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
