Vibrio · Enrichment · granulated
Alkaline Saline Peptone Water – granulated ASPW for Vibrio
The granulated Alkaline Saline Peptone Water with 3 % sodium chloride is used for the enrichment of Vibrio parahaemolyticus and other halophilic vibrios from seafood. Granules produce less dust and dissolve evenly.
- Granulated – less dust, better flow
- 3 % sodium chloride, pH 8.5
- For halophilic vibrios
How Alkaline Saline Peptone Water works
Peptone provides nutrients, and the alkaline pH of about 8.5 together with sodium chloride favours Vibrio, which tolerates alkaline conditions better than most accompanying bacteria. A short incubation of 6–8 hours is enough to multiply the target before plating.
Technical data
| Preparation | 40 g/L; boil to dissolve, autoclave 121 °C 10 min |
| Final pH | 8.5 ± 0.2 |
| Incubation | 35–37 °C, 6–8 h |
| Storage | Dry, dark place; keep the container tightly closed |
| Shelf life | 3 years unopened |
Applications and practical notes
Pathogenic Vibrio species occur in seafood, shellfish and coastal waters and are tested according to ISO 21872-1 and the FDA BAM Chapter 9. Typical applications include:
- Fish, shrimp, oysters and other seafood
- Ready-to-eat seafood products
- Coastal and aquaculture water
- Outbreak investigations
Working with Alkaline Saline Peptone Water: Weigh the granules without grinding them and keep the bottle closed between uses.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About the Vibrio method: ISO 21872-1 describes the detection of potentially enteropathogenic Vibrio parahaemolyticus, V. cholerae and V. vulnificus in seafood and other foods. Samples are enriched twice in Alkaline Saline Peptone Water, plated on TCBS Agar and a second selective medium, and typical colonies are purified on saline agar and confirmed biochemically or by PCR. The FDA BAM Chapter 9 follows a similar scheme. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Alkaline Saline Peptone Water is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Alkaline Saline Peptone Water, please send us a request. With every delivery of Alkaline Saline Peptone Water you receive the lot number and expiry date, and on request we send the available documentation for Alkaline Saline Peptone Water.
Where Alkaline Saline Peptone Water fits in Vibrio testing
- Enrichment – Alkaline Saline Peptone Water (ASPW), 37 °C and 41.5 °C
- Isolation – TCBS Agar plus a second medium: chromogenic Vibrio agar, mCPC or CC agar
- Purification – non-selective saline agar such as T1N1 Agar
- Confirmation – oxidase, biochemical identification, PCR or LAMP
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Alkaline Saline Peptone Water (3 % NaCl), granulated | PRO7629 | 250 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
