Pharmacopoeial media · Sterility test · granulated
Fluid Thioglycollate Medium – pharmacopoeial formulation, granulated
This granulated Fluid Thioglycollate Medium has the pharmacopoeial formulation for the sterility test of sterile products. Granules produce less dust when weighing, which is important in clean preparation areas.
- Granulated – less dust, better flow
- Pharmacopoeial sterility test medium
- Anaerobic and aerobic bacteria
How Fluid Thioglycollate Medium works
Sodium thioglycollate and L-cystine lower the redox potential, and a small amount of agar slows the diffusion of oxygen, so anaerobes grow in the depth of the tube and aerobes near the surface. Resazurin shows oxidation as a pink layer at the top.
Typical growth
| Organism | Growth |
| Clostridium sporogenes | Good growth in the depth |
| Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis | Good growth |
Technical data
| Preparation | 29.3 g/L; boil to dissolve, dispense, autoclave 121 °C 15 min |
| Final pH | 7.1 ± 0.2 |
| Incubation | 30–35 °C (sterility test 14 days) |
| Before use | If the pink oxidised layer exceeds one third of the tube, reheat once in a water bath; discard if the whole tube is pink |
| Storage | Dry, dark place; keep the container tightly closed |
| Shelf life | 3 years unopened |
Applications and practical notes
The sterility test of the European Pharmacopoeia (chapter 2.6.1) and USP <71> uses two media: fluid thioglycollate medium for anaerobic and aerobic bacteria and soya-bean casein digest medium for aerobic bacteria and fungi, each incubated for 14 days. Typical applications include:
- Sterility testing of injectables and sterile products
- Media fill (aseptic process simulation)
- Sterility of medical devices and raw materials
- Growth promotion testing of each batch
Working with Fluid Thioglycollate Medium: Weigh the granules without grinding them and keep the bottle closed between uses, because granules also absorb moisture.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About pharmacopoeial microbiological testing: the European Pharmacopoeia and the USP describe harmonised methods for non-sterile products – microbial enumeration (Ph. Eur. 2.6.12, USP <61>) and tests for specified micro-organisms (Ph. Eur. 2.6.13, USP <62>) – and the sterility test for sterile products (Ph. Eur. 2.6.1, USP <71>). The chapters define the media, incubation conditions and the growth promotion test that each batch of medium must pass with reference strains. COLO.Science offers the media, diluents, neutralisers and QC strains for these tests from one source.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Fluid Thioglycollate Medium is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Fluid Thioglycollate Medium, please send us a request. With every delivery of Fluid Thioglycollate Medium you receive the lot number and expiry date, and on request we send the available documentation for Fluid Thioglycollate Medium.
Where Fluid Thioglycollate Medium fits in pharmacopoeial testing
- Sample preparation – Buffered Sodium Chloride-Peptone Solution, Tryptic Soy Broth and neutralising diluents
- Microbial enumeration – Tryptic Soy Agar (TAMC) and Sabouraud Dextrose Agar (TYMC)
- Specified micro-organisms – enrichment in Tryptic Soy Broth, then MacConkey, Mannitol Salt, Cetrimide, XLD, VRBG, RCM and Columbia media
- Sterility test – Fluid Thioglycollate Medium and Tryptic Soy Broth, 14 days
See all pharmacopoeial media in the Microbiology Catalogue →
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Fluid Thioglycollate Medium (Pharmacopoeia), granulated | PRO7633 | 250 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
