Supplement · Lactose TTC (Tergitol-7) agar
Lactose TTC Agar Supplement – Tergitol-7 and TTC for coliform agar
Lactose TTC Agar Supplement is a two-component supplement for Lactose TTC Agar with Tergitol-7, a membrane filtration medium for coliforms and E. coli in water.
- Two components: Tergitol-7 and TTC
- One A + one B vial per 100 mL
- For membrane filtration of water
How Lactose TTC Agar Supplement works
Sodium heptadecyl sulfate (Tergitol-7) inhibits Gram-positive bacteria, and TTC is reduced by many non-coliforms to a red colour. Coliforms ferment lactose and form yellow-orange colonies with a yellow halo under the membrane.
Technical data
| Preparation | A: sodium heptadecyl sulfate 0.01 g in 1 mL; B: TTC 0.0025 g in 0.5 mL; 1 A + 1 B per 100 mL sterilised base cooled to ~50 °C |
| Storage | 2-8 °C, protected from light after opening |
Applications and practical notes
Membrane filtration and presence-absence media are used for coliforms and E. coli in drinking water, source water and bottled water. Typical applications include:
- Drinking water and source water monitoring
- Bottled water and ice testing
- Swimming pool and process water checks
- Laboratories working with membrane filtration
Working with Lactose TTC Agar Supplement: Add both components to sterilised base cooled to about 50 °C, mix gently and pour at once.
Good practice with media and supplements: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About coliform and E. coli methods: coliforms and E. coli are counted either by the multiple-tube (MPN) method, with a presumptive broth followed by confirmation broths, or by colony counts on selective agars. FDA BAM Chapter 4 describes both approaches for food and water, and ISO 16649-2 gives the colony-count method for β-glucuronidase-positive E. coli on TBX Agar. E. coli O157 and other STEC need dedicated media, serology and PCR. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Lactose TTC Agar Supplement is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Lactose TTC Agar Supplement, please send us a request. With every delivery of Lactose TTC Agar Supplement you receive the lot number and expiry date, and on request we send the available documentation for Lactose TTC Agar Supplement.
Where Lactose TTC Agar Supplement fits in coliform and E. coli testing
- Presumptive test – Lauryl Sulfate Tryptose Broth (MPN) or VRBL / chromogenic agar (colony count)
- Confirmation – BGLB Broth for coliforms; EC or EC-MUG Broth at 44.5 °C for E. coli
- Isolation – EMB, MacConkey, TBX or chromogenic E. coli agar
- Identification – IMViC kit; for O157 and STEC: SMAC or chromogenic O157 agar, latex and PCR
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Lactose TTC Agar Supplement (Tergitol-7) | PRO7712 | 2 × 5 vials |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
COLO LabExperts
Polje ob Sotli 4, SI-3255, Slovenia
Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
COLO.SCIENCE | MICROBIOLOGY SUPPORT
Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
