Coliforms and E. coli · Isolation · granulated
Eosin Methylene Blue Agar – granulated format
The granulated Eosin Methylene Blue Agar has the same use as the powder: isolation and differentiation of coliforms and E. coli. Granules reduce dust and dissolve evenly.
- Granulated – cleaner weighing
- E. coli: green metallic sheen
- Classic completed coliform test
How Eosin Methylene Blue Agar works
Eosin and methylene blue suppress Gram-positive bacteria and indicate lactose fermentation. Strong acid production by E. coli produces dark colonies with a green metallic sheen.
Typical colony appearance
| Organism | Colonies |
| E. coli | Black centre, green metallic sheen |
| Salmonella | Colourless to amber |
Technical data
| Preparation | 37.5 g/L; boil with stirring until dissolved; autoclave 121°C 15 min; pour plates at ~50°C |
| Final pH | 7.1±0.2 |
| Incubation | 35-37°C for 24 h |
| Storage | Dark, dry place |
| Shelf life | 3 years unopened |
Applications and practical notes
Plate count and isolation media for coliforms and E. coli are used in food, feed, water and hygiene testing, for example according to ISO 4832 and ISO 16649-2. Typical applications include:
- Coliform and E. coli colony counts in food and feed
- Isolation of lactose-fermenting Gram-negative bacteria
- Process hygiene criteria in dairy, meat and vegetable production
- Teaching and routine quality-control laboratories
Working with Eosin Methylene Blue Agar: Protect prepared plates from light; methylene blue is light-sensitive.
Good practice with dehydrated media: keep the bottle tightly closed in a cool, dry place, weigh the powder quickly to avoid moisture uptake, and use fresh distilled or deionised water. Check the pH of each new batch after preparation and before use, and label prepared media with the date. Performance of each lot can be verified with reference strains according to ISO 11133, the standard for the preparation, production, storage and performance testing of culture media.
About coliform and E. coli methods: coliforms and E. coli are counted either by the multiple-tube (MPN) method, with a presumptive broth followed by confirmation broths, or by colony counts on selective agars. FDA BAM Chapter 4 describes both approaches for food and water, and ISO 16649-2 gives the colony-count method for β-glucuronidase-positive E. coli on TBX Agar. E. coli O157 and other STEC need dedicated media, serology and PCR. COLO.Science offers media, supplements, rapid tests, identification kits and QC strains for every step.
Complete sets from one supplier: COLO.Science supplies culture media, supplements, rapid tests, identification kits and QC strains as complete sets for the method you follow. Send us your sample types and standards, and we will prepare an offer with the matching products and documentation.
Eosin Methylene Blue Agar is supplied by COLO.Science in the pack sizes listed under Ordering information. For larger quantities, regular deliveries or method-specific sets that include Eosin Methylene Blue Agar, please send us a request. With every delivery of Eosin Methylene Blue Agar you receive the lot number and expiry date, and on request we send the available documentation for Eosin Methylene Blue Agar.
Where Eosin Methylene Blue Agar fits in coliform and E. coli testing
- Presumptive test – Lauryl Sulfate Tryptose Broth (MPN) or VRBL / chromogenic agar (colony count)
- Confirmation – BGLB Broth for coliforms; EC or EC-MUG Broth at 44.5 °C for E. coli
- Isolation – EMB, MacConkey, TBX or chromogenic E. coli agar
- Identification – IMViC kit; for O157 and STEC: SMAC or chromogenic O157 agar, latex and PCR
Ordering information
| Product | COLO code | Pack |
|---|---|---|
| Eosin Methylene Blue Agar (EMB), granulated | PRO7607 | 250 g |
Product notice: preparation, storage conditions and shelf life are given on the label. Technical data on this page are for orientation; the label and the lot documentation apply. B2B prices are quoted net, excluding VAT.
Sales and technical support
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Quotations and technical support for culture media and microbiology products across our international sales territory. B2B prices are net, excluding VAT.
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Need the complete set for your method?
Send the sample type, target organism and the standard you follow. COLO.Science will put together the media, supplements, QC strains and confirmation tests for the whole procedure.
